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pET28_mouse-Caspase-1_enzymatic-domain

Plasmid

#183385

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404) (Casp1 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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MT2a

Plasmid

#105693

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a

Depositor

Artur Krężel

Article

Drozd et al Metallomics. 2018 Apr 25;10(4):595-613.

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mEYFP

Plasmid

#103985

Purpose

Plasmid encoding mEYFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEYFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, V68L, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-moxGFP

Plasmid

#103984

Purpose

Plasmid encoding moxGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean

Plasmid

#103968

Purpose

Plasmid encoding mCerulean

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

ProC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mTFP1

Plasmid

#49644

Purpose

TALETeal-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mTFP1

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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sZF3

Plasmid

#43834

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF3

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-His3MX6-PGAL1-GFP

Plasmid

#41616

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-3HA

Plasmid

#41610

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-His3MX6

Plasmid

#41604

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBN396

Plasmid

#109326

Purpose

Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

Depositor

Peter Askjaer

Article

Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

Insert

mex-5 (mex-5 Nematode)

Expression

Worm

Promoter

mex-5

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS5

Plasmid

#104354

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-sfGFP

Plasmid

#103983

Purpose

Plasmid encoding sfGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

sfGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean3

Plasmid

#103973

Purpose

Plasmid encoding mCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-CLASP2 512-650 9xS/A

Plasmid

#24410

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) 9xS/A (CLASP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant that c…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (E87D)

Plasmid

#29547

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E87D

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only