We narrowed to 80,162 results for: ELL

1. H2B-mGreenLantern

   Plasmid

   #164464

   Purpose

   Localization of mGreenLantern fluorescent protein to histone 2B

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Tags

   Human Histone 2B (D26G and V119I mutations)

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

   Plasmid

   #188385

   Purpose

   Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-mGreenLantern

   Plasmid

   #164469

   Purpose

   Constitutive CAG-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CAG

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pX330-PITCh-MAPRE1

   Plasmid

   #207793

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-CEP135

   Plasmid

   #227286

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. DPYSL2-pLJC2

   Plasmid

   #189866

   Purpose

   Expresses human DPYSL2 (CRMP2) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

   Insert

   DPYSL2 (DPYSL2 Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-Sox2

   Plasmid

   #16353

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-Oct4

   Plasmid

   #16352

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi G418 Hu Her2

    Plasmid

    #183246

    Purpose

    Expression of human HER2 antigen in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

    Insert

    Her2 (ERBB2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmGFP11C-­Tau

    Plasmid

    #71434

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Tags

    GFP (213-228)

    Expression

    Mammalian

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. LifeAct-mGreenLantern

    Plasmid

    #164459

    Purpose

    Localization of mGreenLantern fluorescent protein to actin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102798

    Purpose

    Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    H231L

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmGFP10C-­Tau

    Plasmid

    #71433

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Tags

    GFP (1-212)

    Expression

    Mammalian

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMSCV-FKBP-IRF3

    Plasmid

    #221260

    Purpose

    Expression of IRF3 in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRF3 (Irf3 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-Hhex

    Plasmid

    #131600

    Purpose

    expression of mouse Hhex in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Hhex (Hhex Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rlucrarr3-Flash5

    Plasmid

    #74133

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

    Plasmid

    #214881

    Purpose

    Lentiviral vector encoding RfxCas13d targeting B2M guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crB2M-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid

    #80408

    Purpose

    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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