We narrowed to 59,233 results for: %s

1. pcDNA3-HA-GBF1(E794K)

   Plasmid

   #206416

   Purpose

   Mammalian expression of hGBF1(E794K)

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E794K

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pyc111m

   Plasmid

   #158779

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L309-myc-seipin-WT

   Plasmid

   #64061

   Purpose

   myc-tagged murine seipin (wild type); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET28_6xHis-PARP1delCAT

   Plasmid

   #173942

   Purpose

   Bacterial expression of PARP1 lacking CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delCAT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 661-1014

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ROSA26(MultiFPsΔPuro)

   Plasmid

   #140759

   Purpose

   Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Inserts

   puromycin-N-acetyltransferase
   Venus
   mCherry
   mCerulean
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSpB-mCherry-p63DH

   Plasmid

   #176112

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1delWGR

   Plasmid

   #173940

   Purpose

   Bacterial expression of PARP1 lacking WGR domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-surrogate-reporter

   Plasmid

   #65624

   Purpose

   Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attP
   

   Use

   Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. VPS13C^halo∆1235-1748

    Plasmid

    #187297

    Purpose

    Express VPS13C truncation mutant in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Cai et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS13C truncation mutant (VPS13C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS1928

    Plasmid

    #205967

    Purpose

    JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH all proteins, with His-tagged Cas8
    

    Use

    Tags

    14xHis on Cas8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXNPH3

    Plasmid

    #110098

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. cpGFP-Adhiron_flex

    Plasmid

    #165044

    Purpose

    Flexible linker control for POLArISact expression in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    cpGFP-Adhiron (connected with a flexible linker)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1861

    Plasmid

    #205974

    Purpose

    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-NG-CBE C

    Plasmid

    #227422

    Purpose

    expresses the C-terminal of AAV-NG-CBE

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    AAV-NG-CBE C-terminal
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-U6sgbbBsmbI-puro-2A-Fluc

    Plasmid

    #100277

    Purpose

    Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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