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pBIG2abc

Plasmid

#80617

Purpose

pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 PARL-FLAG-CT S277G

Plasmid

#13615

Depositor

Luca Pellegrini

Publication

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic Serine 277 to Alanine

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Jacquere (all-in-one vector)

Pooled library

#247027

Purpose

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

Depositor

John Doench, David Root

Publication

Drepanos et al Cell Genom. 2026 May 13;6(5):101190. doi

Has service

Lentiviral Prep

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMB67

Plasmid

#47947

Purpose

Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

Depositor

Mike Boxem

Publication

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Promoter

hsp-16.48

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV FLIP p53

Plasmid

#19745

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 (Trp53 Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO-Tet-On-YAP1-shRNA2

Plasmid

#176847

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Publication

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh2 (YAP1 Human)

Use

Lentiviral

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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plRL61

Plasmid

#163633

Purpose

Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

Plasmid

#230803

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

Plasmid

#224006

Purpose

AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has service

AAV8

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12754 - pAAV-AiE0680m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2754)

Plasmid

#224004

Purpose

AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has service

AAV1

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14467 - pAAV-AiE2182m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4467)

Plasmid

#224043

Purpose

AiE2182m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT neurons (strong)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Has service

AAV8

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP11253 - pAAV-eHGT_017h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1253)

Plasmid

#163497

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLS-PC1

Plasmid

#41520

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Polycystin-1 (PKD1 Human)

Tags

Myc/His

Expression

Mammalian

Mutation

sequence from 4107 - 4303 of PKD1

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Publication

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Publication

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/tom20-pbira(79-c)-ha

Plasmid

#154456

Purpose

expresses tom20-pbira(79-c)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Publication

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

tom20-pbira(79-c)-ha (TOMM20 Human)

Expression

Mammalian

Promoter

CMV-F

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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