We narrowed to 11,432 results for: 110

1. pDEST40-mPTER

   Plasmid

   #224864

   Purpose

   Mammalian expression mouse PTER protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   mouse PTER (Pter Mouse)
   

   Use

   Tags

   C-term v5 and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET20b-mPTER

   Plasmid

   #222189

   Purpose

   Bacterial expression of mouse PTER protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   PTER (Pter Mouse)
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221_SLC30A6

   Plasmid

   #132065

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC30A6 (SLC30A6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pQCXIP mScarlet-MOSPD2 RD/LD

   Plasmid

   #186476

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-TOP3B

   Plasmid

   #172423

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PB-Neo-TetO-Cdk12_Nterm_FlagHa

   Plasmid

   #127179

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Mutation

   Promoter

   TetO Promoter

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIP mScarlet-ER [TM(SAC1)]

   Plasmid

   #186572

   Purpose

   Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1 _3xHA-Ago2

   Plasmid

   #73538

   Purpose

   Inducible lentiviral expression of Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBridge-tyrosinase.σ2

   Plasmid

   #197415

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-2 σ2
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   encodes R42G substitution, contains silent substi…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACTB-(A)1x

    Plasmid

    #112055

    Purpose

    ACTB mRNA tagged with 1 copy of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    1 copy of Riboglow tag (variant A) after stop cod…

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT TO GFP11-LRRK2_G2019S

    Plasmid

    #231175

    Purpose

    Expresses GFP-11-LRRK2 G2019S in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (G2019S) (LRRK2 Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-BRPF3

    Plasmid

    #65383

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRPF3 (BRPF3 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. LMPd Ametrine Got2 shRNA#3

    Plasmid

    #220593

    Purpose

    Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

    Insert

    Got2 shRNA (Got2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSems leader-ALFA-mXFP-TpoR

    Plasmid

    #222945

    Purpose

    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    Thrombopoietin receptor (26-635) (MPL Human)
    

    Use

    Tags

    ALFAtag, Ig k-chain leader sequence, and mXFP

    Expression

    Mammalian

    Mutation

    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

    Plasmid

    #212487

    Purpose

    Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn GFP-Fxr1

    Plasmid

    #112732

    Purpose

    AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

    Depositor

    Martin Beaulieu

    Article

    Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

    Insert

    Fxr1p (Fxr1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti GW V5 Eco/Dam mse LaminA

    Plasmid

    #182674

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_122

    Plasmid

    #208095

    Purpose

    Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

    Depositor

    John Doench

    Article

    Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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