We narrowed to 11,122 results for: HA

1. pcDNA-Cas9-T2A-STOP-TdT

   Plasmid

   #126425

   Purpose

   Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Mutation

   alanine-6 of TdT has been changed to a STOP codon

   Promoter

   pCMV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PSD-95 scFv [K28/42]

   Plasmid

   #190488

   Purpose

   Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/42.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Flag-RNF168 delta MIU1

   Plasmid

   #133978

   Purpose

   mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of MIU1

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. INCTbiosyn-p35S-GFP(rc)13

   Plasmid

   #127526

   Purpose

   Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEN-CMV-VPR-WT CjCas9 V-WT

   Plasmid

   #169914

   Purpose

   Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-SpCas9

   Plasmid

   #130968

   Purpose

   3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   3xHA-NLS-SpCas9
   

   Use

   CRISPR

   Tags

   3x HA, EGFP, and NLS

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCB268

   Plasmid

   #53363

   Purpose

   pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

   Depositor

   Alexander Varshavsky

   Article

   Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

   Inserts

   DHFR (Dhfr Mouse)
   Ub
   Aβ42 (APP Human)
   

   Tags

   13xMyc, Flag, and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. BKbeta2 K+ channel scFv [N53/32]

   Plasmid

   #190561

   Purpose

   Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid

    #114092

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (S542R/K548V/N552R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid

    #127527

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. INCTbiosyn-p35S-GFP(rc)Bxb1

    Plasmid

    #127528

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Calbindin scFv [L109/57]

    Plasmid

    #182071

    Purpose

    Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPB-cT3G-cERP2-KLF4

    Plasmid

    #192908

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF4 (KLF4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ankyrin-G scFv [N106/20]

    Plasmid

    #190525

    Purpose

    Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Flag-RNF168 delta MIU2

    Plasmid

    #133979

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU2

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pPB-cT3G-cERP2-JUN

    Plasmid

    #192895

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of JUN

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    JUN (JUN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PSD-95 scFv [K28/43]

    Plasmid

    #190489

    Purpose

    Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/43.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PSD-95 scFv [K28/38]

    Plasmid

    #190487

    Purpose

    Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/38.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge