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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Article

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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p404-BrdU-Inc

Plasmid

#71790

Purpose

BrdU-incorporating plasmid with selectable marker TRP1

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAX

Plasmid

#53274

Purpose

Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

Insert

crtE, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Mutation

Deleted idi (ORF6) and crtX genes

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-rsTagRFP

Plasmid

#31927

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Tags

His

Expression

Bacterial

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

araB

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pPAGFP-TPX2

Plasmid

#31226

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

PAGFP

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
p21 shRNA2

Plasmid

#25869

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

p21 (Cdkn1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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LPCX Smad3 deltaSSVS

Plasmid

#12639

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pJCSUP35(1-253)

Plasmid

#1089

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 253 of SUP35

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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CD8-GFP-FKBP

Plasmid

#100751

Purpose

Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

CD8A (CD8A Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_SEPT9 i4

Plasmid

#71617

Purpose

mammalian expression of human SEPT9 i4 with a GFP fusion

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_SEPT9 i1

Plasmid

#71609

Purpose

mammalian expression of human SEPT9 i1 with a GFP fusion.

Depositor

Cristina Montagna, Elias Spiliotis

Article

Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

Insert

Septin 9 (SEPTIN9 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-544

Plasmid

#53548

Purpose

mammalian expression of TPP1 full length

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only