165,906 results

1. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170810

   Purpose

   Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_SMURF2_WT

   Plasmid

   #82259

   Purpose

   Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMURF2 (SMURF2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALED_Right-mouse-ATP6-1397C-AD

   Plasmid

   #212954

   Purpose

   Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

   Insert

   TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac-GFP-dynein-2(D1091–Q4307)

   Plasmid

   #64064

   Purpose

   Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

   Depositor

   Andrew Carter

   Article

   Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

   Insert

   Cytoplasmic dynein-2 (DYNC2H1 Human)
   

   Use

   Tags

   GFP, His tag, and ZZ tag

   Expression

   Insect

   Mutation

   Deleted amino acids 1-1090, extra C-terminal vali…

   Promoter

   Polyhedrin

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJWV102-wtcas9sp

   Plasmid

   #99612

   Purpose

   Plasmid containing wild-type cas9sp

   Depositor

   Jan-Willem Veening, Morten Kjos

   Article

   van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

   Insert

   cas9sp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PczcD

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. peGFP C1-SPG20 SC domain

   Plasmid

   #218580

   Purpose

   Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-417

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_DYNLL1_Dynein-light

   Plasmid

   #110006

   Purpose

   Protein expression and purification of DYNLL1_Dynein-light

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DYNLL1_Dynein-light (DYNLL1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TT 3xFLAG-TWIST2

   Plasmid

   #197935

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-ZIKVA-GFP-puro

    Plasmid

    #140090

    Purpose

    Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    ZIKVA-GFP
    

    Use

    Lentiviral; Low expression

    Tags

    6XHis tag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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