We narrowed to 14,170 results for: ef1a

1. pLenti-EF1a-Ly6G-miR30

   Plasmid

   #163358

   Purpose

   Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   Ly-6G (Ly6g Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-Haunt-EF1a-DsRed

   Plasmid

   #92162

   Purpose

   Haunt in trans overexpression plasmid

   Depositor

   Xiaohua Shen

   Article

   Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

   Insert

   Haunt (Halr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPD1026 EF1alpha TetON3G in TU2

   Plasmid

   #139258

   Purpose

   Expresses TetON3G under hEF1alpha in TUPV2

   Depositor

   Joshua Leonard

   Insert

   TetON3G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1alpha

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP251 pEF1a-UNC5C-4-SunTag

   Plasmid

   #100940

   Purpose

   TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   TALE target sequence for UNC5C, SunTag array (10xGCN4)
   

   Use

   TALEN

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

   Plasmid

   #167826

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. miniTol2 x EF1a_EKAREN4 + PGK-blast

   Plasmid

   #167825

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

   Plasmid

   #138316

   Purpose

   Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   SpyCas9 GFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLENTI EF1alpha FLAG/HA-LCOR

   Plasmid

   #114444

   Purpose

   Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   LCOR (LCOR Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV40-EF1a-hBCL10(1-185)-SO

   Plasmid

   #75258

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   AS 1-185 (w/o C-terminus)

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP245 pEF1a-TALE-DNMT3a-Wt

    Plasmid

    #100937

    Purpose

    Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    TALEN

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP338 pEF1a-DACH1-1-SunTag

    Plasmid

    #100942

    Purpose

    TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE target sequence for DACH1, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-Ef1a-SOX17WT-3xFLAG

    Plasmid

    #216198

    Purpose

    Overexpress wild-type human SOX17 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-Ef1a-SOX17E57D-3xFLAG

    Plasmid

    #216199

    Purpose

    Overexpress human SOX17E57D in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE2-Ef1a-SOX17E57P-3xFLAG

    Plasmid

    #216200

    Purpose

    Overexpress human SOX17E57P in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE2-Ef1a-OCT4-3xFLAG

    Plasmid

    #216196

    Purpose

    Overexpress human OCT4 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

    Plasmid

    #195567

    Purpose

    PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

    Depositor

    Joanna Wysocka

    Article

    Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

    Insert

    TIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-hU6-2O-crBFP_EF1a-BFP

    Plasmid

    #224783

    Purpose

    BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC-hU6-2O-crEGFP_EF1a-BFP

    Plasmid

    #224784

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-EF1aL-E690Kmut_ABCA3-GFP

    Plasmid

    #188549

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid E690->K690 (ABCA3 gene)

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-EF1aL-W308Rmut_ABCA3-GFP

    Plasmid

    #188550

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3 (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid W308->R308 (ABCA3 gene)

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPB-EF1a-HOXB5-DD-hygro

    Plasmid

    #222582

    Purpose

    PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a Promoter (MTK 2) (pAN2824)

    Plasmid

    #194225

    Purpose

    Mammalian Toolkit part 2 containing the EF1a promoter

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221487

    Purpose

    NKX2-1 N-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge