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pMaCTag-09

Plasmid

#119988

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_minPGK

Plasmid

#62415

Purpose

MXS_chaining vector with minPGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-29

Plasmid

#120008

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23102_P

Plasmid

#126997

Purpose

Basic Part P- Promoter (constitutive); J23102.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23102_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP39-1A(sfGFP)

Plasmid

#126983

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-04

Plasmid

#119983

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SaCas9_sgRNA2

Plasmid

#107330

Purpose

U6 driven SaCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-08

Plasmid

#119987

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_SV40pA

Plasmid

#62426

Purpose

MXS_chaining vector with SV40pA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

SV40 poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_C

Plasmid

#127036

Purpose

Basic Part C- C-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMVenhancer

Plasmid

#62419

Purpose

MXS_chaining vector with CMVenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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