We narrowed to 2,178 results for: ipp

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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS L4137P-GST

Plasmid

#83455

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, L4137P

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS R4150C-GST

Plasmid

#83454

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, R4150C

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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tdEos-Cx43-7

Plasmid

#57603

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Cx43

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUASp-Flag-Myc-Qin-Tudor-attB

Plasmid

#44210

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin-Tudor-domain (qin Fly)

Use

Fly transformation

Tags

Flag and Myc

Mutation

lacks E3 domain on N-term (del aa1-274)

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-His3MX6-PGAL1-GST

Plasmid

#41613

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

Plasmid

#179716

Purpose

Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-A'Q-mito

Plasmid

#158766

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-'Q-mito

Plasmid

#158767

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pKL123

Plasmid

#187889

Purpose

AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

Depositor

Martin Kampmann

Article

Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

Insert

NFIA-SOX9

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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HOP-flash

Plasmid

#83467

Purpose

TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/cyto-iAspSnFR

Plasmid

#201394

Purpose

CMV overexpression of cyto-iAspSnFR in mammalian cell lines

Depositor

Kai Johnsson

Article

Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

Insert

cyto-iAspSnFR

Expression

Mammalian

Mutation

gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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