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LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

Plasmid

#219828

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-GFP-WPRE

Plasmid

#211488

Purpose

expresses GFP under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_PUUV_GP

Plasmid

#207301

Purpose

Mammalian expression of Puumala virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PUUV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_LACV_GP

Plasmid

#207286

Purpose

Mammalian expression of La Crosse virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LACV_GP (M La Crosse virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_HCV_E1E2

Plasmid

#207279

Purpose

Mammalian expression of Hepatitis C virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUCV_HE

Plasmid

#207275

Purpose

Mammalian expression of Influenza C virus HE protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUCV_HE

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-8xPP7

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP-mTLN1-FL

Plasmid

#164838

Purpose

Expression of GFP tagged FL mouse talin1

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28c-Ku

Plasmid

#161936

Purpose

Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

Depositor

Harris Wang

Article

Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

Insert

ku (mku Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-FKBP

Plasmid

#128771

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FKBP

Expression

Mammalian

Available Since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pTEF6-7

Plasmid

#124583

Purpose

Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

mCherry

Expression

Yeast

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1306

Plasmid

#111128

Purpose

Expresses FANCE.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCE (FANCE Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pTorPE-GR-GECO1.2

Plasmid

#42199

Depositor

Robert Campbell

Article

Hoi et al J Am Chem Soc. 2012 Dec 26.

Insert

GR-GECO1.2

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pPD95.77-wVenus

Plasmid

#37465

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco S239A

Plasmid

#112194

Purpose

Expression of MCPyV LT mutant (Ser 239 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.28TM.28.3z

Plasmid

#200674

Purpose

Modular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD28TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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sAB-MBP

Plasmid

#204736

Purpose

synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment recognizing bacteria maltose-binding protein

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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