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PF10_0166-bio-His

Plasmid

#50801

Purpose

Expresses enzymatically monobiotinylated full length PF10_0166

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF10_0166

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP-miR-223 sponge

Plasmid

#97150

Purpose

For miR-223 knock down

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223 sponge

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA mouse NHERF-1 (1-325)

Plasmid

#32706

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)

Tags

none

Expression

Mammalian

Mutation

Deleted last 30 AA putative Ezrin binding site

Promoter

CMV

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4-Tet-on-vsv-Incenp

Plasmid

#108480

Purpose

inducible expression of vsv-INCENP wt

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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PGL3-Ogt (-178 to +233 bp)

Plasmid

#79266

Purpose

To identify the Cis and Trans acting elements that control Ogt gene transcription.

Depositor

Steven Jones

Article

Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

Insert

Ogt promoter element (Ogt Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-Plxnb3-ISH-2kb

Plasmid

#68037

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pWPXL-resNP1

Plasmid

#65061

Purpose

Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

G447A, C450T, A451T, G452C, C454A, C456A, C459G

Promoter

EF1alpha

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4-IGd2

Plasmid

#22536

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 4 (HDAC4 Human)

Tags

IGd2 = Ires-destabilized GFP

Expression

Mammalian

Available Since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5 713

Plasmid

#65246

Purpose

Expression of HA tagged ERK5 AA 2-713 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 713 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 714-817

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4g-ISH-2kb

Plasmid

#68034

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4G (Sema4g Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FCIV1-GR SS287AA/S155A

Plasmid

#72700

Purpose

Lentiviral expression of GR-2A phosphodeficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA/S155A

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA4b delta6

Plasmid

#47590

Depositor

Emanuel Strehler

Article

Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

Insert

PMCA4bdelta6 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only
pBS BRD7

Plasmid

#19232

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Bromodomain containing 7 (BRD7 Chicken)

Expression

Bacterial

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(C-domain)

Plasmid

#83518

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Tags

GB1 and His

Expression

Bacterial

Mutation

C domain (contains residues 340-399)

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pBS TMEM108

Plasmid

#18852

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Hypothetical protein, Tmem108 (HAPLN1 Chicken)

Expression

Bacterial

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only