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pcDNA3.2/V5 hsa-mir-124

Plasmid

#26306

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-124 (MIR124-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pFX-iRFP-Rab7

Plasmid

#174459

Purpose

Express iRFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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YF-Cdc42(CA)

Plasmid

#20152

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Cdc42(CA) (CDC42 Human)

Expression

Mammalian

Mutation

Cdc42H was inserted into the multiple cloning sit…

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only

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pCI-neo:alpha4-mcherry

Plasmid

#45073

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

alpha4-mcherry (Chrna4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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TIMP3-bio-His

Plasmid

#53418

Purpose

Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TIMP3 (TIMP3 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
NAX_CAGGS_CAS9_m4_VPR_KanR neo

Plasmid

#167885

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSN838

Plasmid

#184826

Purpose

Expresses human KIF5A(∆exon27) in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

∆exon27 form

Promoter

CMV

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-M3(T3D)471-721

Plasmid

#56657

Purpose

GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing

Depositor

Max Nibert

Article

Broering et al J Virol. 2005 May;79(10):6194-206.

Insert

M3(T3D)421-721

Tags

EGFP

Expression

Mammalian

Mutation

insert encodes aa 471-721 + native stop codon of …

Promoter

CMV-IE

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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YFP-Rac1(N17)

Plasmid

#11395

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 mutated to Asparagine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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mCerulean3-ER-5

Plasmid

#55418

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

ER (CALR Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAT9650-BEAR-GFP-preedited

Plasmid

#162992

Purpose

BEAR control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-p97-E578Q

Plasmid

#85672

Purpose

Expression of human p97 E578Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Tags

GFP

Expression

Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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(318) pcDNA3.1-myc-OGA(1-400)D174N

Plasmid

#168096

Purpose

Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

myc-OGA(1-400, D174N)

Tags

myc

Expression

Mammalian

Mutation

D174N catalytically inactive OGA

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cerulean-GalT

Plasmid

#11930

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
ScFv-2ERT2-VPH

Plasmid

#120556

Purpose

Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

scFvGCN4, GB1, ERT2, VP64, P65 and HSF1

Use

Lentiviral

Expression

Mammalian

Promoter

hPGK

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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