We narrowed to 13,693 results for: crispr cas9

1. pYPQAT58

   Plasmid

   #223430

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT61

   Plasmid

   #223433

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDB_182

   Plasmid

   #216103

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNICKclos2.0

   Plasmid

   #73228

   Purpose

   Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sgRNA to xylR
   

   Use

   E.coli - clostridium shuttle vector

   Mutation

   D10A

   Promoter

   Pj23119 and Pthl

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDB_181

   Plasmid

   #216102

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_662

   Plasmid

   #216101

   Purpose

   CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330 Ctnnb1.2

   Plasmid

   #59912

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-sgCCR5

    Plasmid

    #83930

    Purpose

    Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCCR5
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AcrIIC1X

    Plasmid

    #128112

    Purpose

    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX (AcrIIC1 N3F/D15Q/A48I)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDR217

    Plasmid

    #113872

    Purpose

    expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA9-MPH2-3 sgRNA10-MAL11
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_GABPB1_iso2_1

    Plasmid

    #104041

    Purpose

    Encodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    GABPB1_iso2 gRNA (GABPB1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pURD214

    Plasmid

    #113871

    Purpose

    expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA5 HXT13-15-16 sgRNA2-HXT2
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDR220

    Plasmid

    #113873

    Purpose

    expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA8-HXT10 sgRNA7-HXT9-11-12
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUDR418

    Plasmid

    #113875

    Purpose

    expression of double Cas9 programming sgRNA11 targetting STL1

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    doble sgRNA11-STL1
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB9P3

    Plasmid

    #210034

    Purpose

    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-sgALCAM

    Plasmid

    #83929

    Purpose

    Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgALCAM
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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