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pCS2 Flag Smad3 EPSM A208S

Plasmid

#27112

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; A208S

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Human T (BRACHYURY) sgRNA

Plasmid

#59726

Purpose

Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

hT sgRNA (TBXT Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
pTYF-super-mfPENK-2.2k-Venus-WPRE

Plasmid

#156400

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 EPSM A213S

Plasmid

#27113

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; A213S

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pSN65

Plasmid

#100123

Purpose

expresses putative SOX2 DBD with a V5 tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSERT-1.9k-Venus-WPRE

Plasmid

#156394

Purpose

Expresses Venus under SERT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b-GB

Plasmid

#129689

Purpose

express E. coli GreB

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

his6/thrombin/GreB (greB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Sa sgRNA expression vector

Plasmid

#107720

Purpose

For in vitro transcription of Sa sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSN20

Plasmid

#100105

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52543

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 NL WT

Plasmid

#27040

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A204S

Plasmid

#27111

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V S204A S208A S213A; A204S

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52547

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4_FUS_C2G_R521G

Plasmid

#26369

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Mutation

R521G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A204S

Plasmid

#27108

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A204S

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
LEPG_shSik3.3974

Plasmid

#125855

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.308

Plasmid

#125878

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge