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Plasmid#134862PurposeExpresses EGFP-tagged Golgi markerDepositorInsertbeta 1,4-galactosyltransferase (B4GALT1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 83-398PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin
Plasmid#134864PurposeExpresses EGFP-tagged nuclear envelope markerDepositorAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
hyeA3A-BE4max
Plasmid#157944PurposeMammalian expression of hyeA3A-BE4maxDepositorInserthyeA3A-BE4max
UseCRISPRExpressionMammalianMutationN57GAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMALB
Plasmid#161786PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsTagBFPExpressionMammalianPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GAVPO
Plasmid#159923PurposeGAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133DepositorInsertGAVPO
ExpressionMammalianPromoterCMVAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_3xFLAG_V5_ eGFP_V5
Plasmid#160111PurposeExpresses eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTags3xFLAG, V5ExpressionMammalianAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.2_3xFLAG_V5_ mmDdx58_V5
Plasmid#160108PurposeExpresses murine Ddx58 in mammalian cellsDepositorAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Cytbow
Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
TagsThe default expressed EBFP2 is fused to H2BExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP
Plasmid#158654Purposemaking transgenics expressing a QUAS driven YFP and NTR 2.0DepositorInsert5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)
Viral prep#112677-AAV9PurposeReady-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-Hygro
Plasmid#158555PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
TagsGSTExpressionBacterialAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-ZNF598
Plasmid#141191PurposeExpresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell lineDepositorAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEL031
Plasmid#137900PurposeSTU2.0 SpyCas9 tRNA system for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMuLE_ENTR_TOP-iRFP_L5-L4
Plasmid#113866PurposeSingle pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
iRFP713
UseGateway CloningExpressionMammalianAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
IC65 pEGFPN1 anti-IRSp53 nanobody
Plasmid#135227PurposeMammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only