We narrowed to 59,942 results for: %s

1. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

   Plasmid

   #105643

   Purpose

   Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

   Depositor

   Sandra Rieger

   Article

   Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

   Insert

   Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pClneoMyc human MST2

   Plasmid

   #37022

   Depositor

   Yutaka Hata

   Article

   Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

   Insert

   MST2 (STK3 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pACEBac1-PSMB5-PSMB6-PSMB7

   Plasmid

   #217898

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB5 (PSMB5 Human)
   PSMB6 (PSMB6 Human)
   PSMB7 (PSMB7 Human)
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP-FKBPx5-His

   Plasmid

   #103785

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-FKBPx5-His
   

   Tags

   EYFP, His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. ePB-PURO-FLAG-METTL3

   Plasmid

   #160252

   Purpose

   For stable integration of FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. attB-­GFP-­Poly(A)

   Plasmid

   #65524

   Purpose

   Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCPam

   Plasmid

   #195861

   Purpose

   Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Amber Chromogenic Protein on pRCPam plasmid
   

   Expression

   Bacterial

   Mutation

   Gln62X

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120297

    Purpose

    AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120298

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pHS1929

    Plasmid

    #205968

    Purpose

    JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Expression

    Mammalian

    Promoter

    CMV-F, T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-hPPP3R1

    Plasmid

    #179136

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-bArr1

    Plasmid

    #201491

    Purpose

    Expression of the beta-arrestin1 containing a N-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    V5-bArr1 (ARRB1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TIA1 RRM-YFP-SspBx6

    Plasmid

    #103781

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-YFP-SspBx6
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-ubiquitin-SV40-RFP

    Plasmid

    #65448

    Purpose

    Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-RFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBQ

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pHS1477

    Plasmid

    #205970

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH all proteins, with His-tagged Cas11
    

    Tags

    14xHis on Cas11

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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