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pEMB018 / LRRK2_BC_1847_2350

Plasmid

#40533

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis

Mutation

WT; aa1847_2350

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pSAC200

Plasmid

#187911

Purpose

psac200-crispey-bar-vector

Depositor

Hunter Fraser

Article

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL3-TRE-MCS-polyA-2L

Plasmid

#11719

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 EGFP-Sumo2 GG

Plasmid

#13384

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 GG (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Mutation

Mature SUMO2GG: diglycine at amino acid positions…

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCOLA-T7-YhjH

Plasmid

#79165

Purpose

Expresses YhjH under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

yhjH

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII

Plasmid

#134355

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

CroAct3 63aa

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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Ube2c

Plasmid

#31418

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2C Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCNPpo6

Plasmid

#46086

Depositor

Raymond Monnat

Article

Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

Insert

PpoI

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)EYFP

Plasmid

#31306

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)EYFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pBbS5c-Maqu_3494

Plasmid

#86205

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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Pgr_opsin

Plasmid

#107655

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

opsin

Use

RNAi; In situ hybridization

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pJRK136

Plasmid

#173744

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 alcSynth-34S

Plasmid

#153411

Purpose

Ethanol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

alcSynth-34S promoter

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMU102

Plasmid

#61805

Purpose

Cloning and expression vector for Clostridium thermocellum

Depositor

Lee Lynd

Article

Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pK

Plasmid

#64188

Purpose

harbours marker gene LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRRL.hPGK.GP91

Plasmid

#30477

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

hPGK/GP91

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TO-DsRed

Plasmid

#16340

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

DsRed

Expression

Mammalian

Mutation

5'Prime Site: Not I 3'Primer Site : No…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only