We narrowed to 19,044 results for: Met

Showing: 10721 - 10740 of 19044 results

Results per page:

Sort results by:

GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
KHBD00486

Plasmid

#39591

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5965 (woc Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pM117: VEGFA array presgRNA

Plasmid

#166869

Purpose

U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

Human VEGFA targeting array presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CRISPR V2 sgShmt2_2

Plasmid

#106315

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting mShmt2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

Plasmid

#117909

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R98A on eDHFR

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPglu-600uDelta13 Aphrodite

Plasmid

#13570

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC myc-mRAP1 I312R

Plasmid

#73137

Purpose

Expression of myc-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Tags

myc

Expression

Mammalian

Mutation

isoleucine changed to an arginine at amino acid 3…

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-His6-OAZ-GSY-SPM-Ctag

Plasmid

#163613

Purpose

Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

OAZ-GSY-SPM

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00359

Plasmid

#34479

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7839 (CG7839 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
OphA-delta C6

Plasmid

#127679

Purpose

Bacterial expression of His-tagged OphA-delta C6

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6

Tags

His8

Expression

Bacterial

Mutation

delta C6

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
As1

Plasmid

#123159

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J117, ParsR, PhrpLE

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MolBC

Plasmid

#92210

Purpose

Expression of H. influenzae MolBC (ABC Transporter) in E. coli

Depositor

Douglas Rees

Article

Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

Inserts

MolC (HI1471 )
MolB (HI1470 )

Tags

8x Histidine Tag and Enterokinase Cleavage Site b…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Neo-PTEN

Plasmid

#16411

Depositor

Bert Vogelstein

Article

Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

Insert

PTEN Neo targeting (PTEN Human)

Use

AAV; Adeno-associated viral

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-FLAG-CaMKKbeta rat K193A

Plasmid

#32450

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

K193A (S3V, G96R, and P108L also present)

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00678

Plasmid

#39725

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7372 (CG7372 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.0_nucLAMA-G97 IRES mYFP

Plasmid

#130713

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS IRES mYFP

Tags

NLS and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Antares-SEPLuc

Plasmid

#141086

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-EMCV IRES-SEPLuc

Expression

Mammalian

Mutation

Deletion of amino acids 66 to 91 on the Luciferas…

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Expression

Yeast

Promoter

His

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge