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pBait-YY1

Plasmid

#165147

Purpose

Bait plasmid for PROBER with YY1-binding site (positive control)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

3X YY1 motif

Use

Unspecified

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pPI227

Plasmid

#113231

Purpose

safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Safe haven knock-in plasmid for the act5 locus,li…

Tags

GFP

Promoter

act5

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mH2A1.2-CT-MYC

Plasmid

#45168

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

HIS and MYC

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pACS142

Plasmid

#204598

Purpose

Histone H3.3K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human H3.3 K9M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K9M - V5

Expression

Mammalian

Mutation

H3.3 K9M

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL MND WASp

Plasmid

#36248

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

WASp (WAS Human)

Use

Lentiviral

Promoter

MND

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-JEDI-1P-Kv-WPRE

Plasmid

#202614

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eLACCO1.1

Plasmid

#168788

Purpose

Neuronal expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLACCO1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

hSyn

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TSen

Plasmid

#209916

Purpose

A FRET biosensor for ADAM17 activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TSen

Tags

ECFP and YPet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-WPRE

Plasmid

#202613

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-AID

Plasmid

#61926

Purpose

Expression of human AID tagged with EGFP

Depositor

Jon Houseley

Article

Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

Insert

AICDA (AICDA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SL016: AAV-CAG-FLEX-CheRiff-GFP

Plasmid

#124776

Purpose

For AAV based expression of CheRiff in Cre-Positive cells

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

CheRiff-GFP

Use

AAV and Cre/Lox

Tags

GFP

Promoter

CAG

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only
GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Mutation

D463N

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-C16orf80-flag

Plasmid

#70645

Purpose

Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C16orf80 (CFAP20 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSFFV-dNme2Cas9

Plasmid

#193190

Purpose

Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

dNme2Cas9

Tags

NLS and NLS-3xHA-NLS

Expression

Mammalian

Mutation

H588A, D16A

Promoter

SFFV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin beta1-tev-BHCys

Plasmid

#27314

Depositor

Timothy Springer

Article

Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

integrin beta1(1-708)+GG+TEV+GGKN+BHCys

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only