We narrowed to 23,036 results for: his
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Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAPRE1
Plasmid#31589DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Mouse 1-87)
Plasmid#62061PurposeExpression of Mouse p53 (residues 1-87) in e. coliDepositorInsertp53 (Trp53 Mouse)
TagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GTF2F2
Plasmid#31323DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG_withTEVsite
Plasmid#124940PurposeExpression of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHisExpressionBacterialMutationwith TEV site between RGG domainsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
D5_Ld_280600
Plasmid#135821PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertmajor surface protease gp63, putative,leishmanolysin, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(515-710)-HinB
Plasmid#35053DepositorInsertIFI16-(515-710)-HinB (IFI16 Human)
TagsHisExpressionBacterialMutationContains HinB domain: aa 515-710PromoterT7Available SinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA
Plasmid#60824Purposeto purify human ITPA from bacteriaDepositorAvailable SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-KCNIP2
Plasmid#34799DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pProEX-Af2Sir2
Plasmid#8380DepositorTagsHisExpressionBacterialAvailable SinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_190-303
Plasmid#118822PurposeBacterial expression of hnRNPA2 truncation (residues 190 to 302).DepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis tag and TEVExpressionBacterialMutationcontains amino acids 190-302Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
Plasmid#56658Purposeexpresses constitutively active human PKCi with a deletion on the PB1 domainDepositorInsertHuman PKCi (PRKCI Human)
UseLentiviralTagsV5/HisMutationA120E and deletion of PB1 domain (aa 24–98)PromoterCMVAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHis and mCherryExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-AK1
Plasmid#31290DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA
Plasmid#35052DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
TagsHisExpressionBacterialMutationContains HinA domain: aa 192-393PromoterT7Available SinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
TagsHisExpressionBacterialPromoterT7Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-luc-TimRA
Plasmid#33133DepositorInsertTim-RA 3' UTR (tim Fly)
UseLuciferaseTagsFirefly LuciferaseExpressionInsectPromoterActin 5CAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJS278
Plasmid#134139PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only