We narrowed to 6,458 results for: chia

1. pJOE6090.1

   Plasmid

   #135080

   Purpose

   pBR322-based L-rhamnose-inducible vector.

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

   Insert

   P-rhaP-BAD-Strep-tag II-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBW-m4046768

   Plasmid

   #145763

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pC002 - LshC2C2 locus into pACYC184

   Plasmid

   #79151

   Purpose

   Endogenous LshC2c2 locus cloned into pACYC184

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBW-m4046754

   Plasmid

   #145647

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. HA-EcPPK1

   Plasmid

   #159279

   Purpose

   Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

   Insert

   HA-tagged Polyphosphate Kinase 1
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS AluYa5rescueA70D

   Plasmid

   #78657

   Purpose

   Generates Alu inserts suitable for rescue

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

   Insert

   Ya5rescue cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046910

   Plasmid

   #145672

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pZ003 (LshC2c2 locus)

   Plasmid

   #70168

   Purpose

   Expresses LshC2c2 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LshC2c2 full locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAJT430

    Plasmid

    #202291

    Purpose

    RPU integration plasmid (LPattB7)

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    J23101-RiboJ00-B0034-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZH520

    Plasmid

    #102673

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZH521

    Plasmid

    #102674

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZH517

    Plasmid

    #102670

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAT.015

    Plasmid

    #119558

    Purpose

    Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAJT254-litR

    Plasmid

    #202308

    Purpose

    litR repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    litR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAJT249-psrA

    Plasmid

    #202304

    Purpose

    psrA repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    psrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-NtC60αC20NtLC60β

    Plasmid

    #160877

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: T7, chaperones: T7

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-Flag-HA-Merlin

    Plasmid

    #27104

    Depositor

    Filippo Giancotti

    Article

    Li et al Cell. 2010 Feb 19. 140(4):477-90.

    Insert

    Merlin (Nf2 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGBW-m4046806

    Plasmid

    #145746

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pSZT025

    Plasmid

    #140033

    Purpose

    KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZH519

    Plasmid

    #102672

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZH522

    Plasmid

    #102675

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS4

    Plasmid

    #55751

    Purpose

    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-59 of KanR

    Promoter

    T7-lac

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST-hPDI

    Plasmid

    #60555

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDI

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pGBW-m4046879

    Plasmid

    #149238

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGBW-m4046942

    Plasmid

    #145757

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pGBW-m4046763

    Plasmid

    #145606

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pRK5-Merlin-Flag-HA

    Plasmid

    #27103

    Depositor

    Filippo Giancotti

    Article

    Li et al Cell. 2010 Feb 19. 140(4):477-90.

    Insert

    Merlin (Nf2 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

37. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAL612

    Plasmid

    #119860

    Purpose

    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysterine 290 to Serine and Glycine 244 to Cystei…

    Promoter

    lac

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pGBW-m4046933

    Plasmid

    #145592

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGBW-m4136463

    Plasmid

    #149527

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pGBW-m4046764

    Plasmid

    #145673

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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45. pSA10(gfcB-His-CD)

    Plasmid

    #176194

    Purpose

    expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcBCD
    

    Use

    Tags

    GfcB-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBH31(gfcD)

    Plasmid

    #176199

    Purpose

    Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBH31(gfcD-10xHis)

    Plasmid

    #176200

    Purpose

    Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pETM6-Pc4CL-CmCHS-MsCHI

    Plasmid

    #73398

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pC0.167

    Plasmid

    #119636

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    non functional/large non-specific insertion

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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