We narrowed to 1,145 results for: cas12

1. pTol2-HybridBarcode

   Plasmid

   #174037

   Purpose

   For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   CRISPR target sites
   

   Use

   Tol2 transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIBR005

   Plasmid

   #177668

   Purpose

   Constitutive expression of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIBR001

   Plasmid

   #177628

   Purpose

   SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSplit-SWITCHER1-TF1-dsRED

   Plasmid

   #213044

   Purpose

   A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

   Depositor

   Petr Bartunek

   Article

   Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

   Insert

   Split-SWITCHER1-TF1-dsRED
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cBH

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSplit-SWITCHER2-TF1-dsRED

   Plasmid

   #213045

   Purpose

   A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

   Depositor

   Petr Bartunek

   Article

   Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

   Insert

   Split-SWITCHER1-TF1-dsRED
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

   Plasmid

   #188274

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   PlmCasX(T26R/K610R/K808R)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1300_pUB10_pcoCASphi_E9t_MCS_version1

   Plasmid

   #197937

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTarget_PS_VB

   Plasmid

   #186456

   Purpose

   pTarget containing protospacer for AaCas12b targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTarget_PS_VA

   Plasmid

   #186454

   Purpose

   pTarget containing protospacer for FnCas12a targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid

    #89351

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. LbCpf1-2NLS

    Plasmid

    #102566

    Purpose

    bacterial expression of LbCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    LbCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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