We narrowed to 3,151 results for: eed

1. pCS2+-FLAG3-hsAgo2D-A

   Plasmid

   #221720

   Purpose

   Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2D-A (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D669A in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-FLAG3-hsAgo2

   Plasmid

   #221719

   Purpose

   Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+-FLAG3-drAgo2Y-F

   Plasmid

   #221715

   Purpose

   Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2Y-F (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Y680F in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+-FLAG3-drAgo2D-E

   Plasmid

   #221714

   Purpose

   Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2D-E (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D651E in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX552 EF1a DIO Chronos GFP_Vgat gRNA

   Plasmid

   #215277

   Purpose

   Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Vgat gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

   Plasmid

   #215278

   Purpose

   Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Vgat control gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

   Plasmid

   #215281

   Purpose

   Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Grin1 gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. H2B-GFP-INCENP∆cen

   Plasmid

   #184044

   Purpose

   Expresses H2B-GFP fused to INCENP∆cen

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   GFP and H2B

   Expression

   Mammalian

   Mutation

   Truncated to 1-862

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mKO2-Parkin

   Plasmid

   #213544

   Purpose

   Transient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Parkin(E16A)

    Plasmid

    #213546

    Purpose

    Transient mammalian expresssion of E16A Parkin

    Depositor

    Lani Wu, Steven Altschuler

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E16A mutation in Parkin

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMHE-MmCENP-O-GFP

    Plasmid

    #205178

    Purpose

    Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Wild type mouse CENP-O

    Promoter

    T7

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. L_NLS-mutNLS V5-Twist

    Plasmid

    #201381

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A4-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M_NLS-mutNLS V5-Twist

    Plasmid

    #201379

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A5-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mutNLS V5-Twist

    Plasmid

    #201380

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H_NLS-mutNLS V5-Twist

    Plasmid

    #201378

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UL_NLS-mutNLS V5-Twist

    Plasmid

    #201382

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    (NLS)SV40A3-V5

    Expression

    Mammalian

    Mutation

    K38R K73R

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mut GBP2 V5-Twist

    Plasmid

    #201377

    Purpose

    Expresses Twist mutant for nucleocytoplasmic transport studies

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Y107E

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

    Plasmid

    #186703

    Purpose

    Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

    Plasmid

    #172582

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD2

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    A. thaliana egg cell promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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