We narrowed to 14,414 results for: TIM;
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Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT-mCitrineV163A-HIS
Plasmid#64685PurposepKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.DepositorInsertmCitrine V163A
ExpressionYeastMutationValine 163 changed to alanineAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARγ
Plasmid#119915PurposeMammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARγ
ExpressionMammalianAvailable SinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM532
Plasmid#112529PurposepZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpRDepositorInsertHrtR
ExpressionBacterialPromoterProDAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTL472
Plasmid#69882PurposedCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassetteDepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG56A -myc
Plasmid#107459PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
Plasmid#89994PurposeDonor template for generation of NGN3-ntdTomato reporter cell linesDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR35
Plasmid#69256Purposephsp16.41::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.41Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F463A
Plasmid#45975DepositorInsertParkin F463A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR28
Plasmid#69253Purposephlh-8::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhlh-8Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
TagsmYPetPromotermex-5Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291
Plasmid#129671PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneExpressionPlantMutationBthCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
Plasmid#124604PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulationDepositorInsertSoma-localized C1V1(t/t)
UseAAVTagsmRuby2ExpressionMammalianPromoterEf1aAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …ExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only