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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,240 results for: BASE

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Showing: 10801 - 10820 of 13240 results

  3. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid
    #171660
    Purpose
    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171660-plasmid-map-sequence-id-339032

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  10. PXWABS62(E11-TEV)

    Plasmid
    #123157
    Purpose
    For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ParsR-ABS62, Pecf11
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123157-plasmid-map-sequence-id-243988

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  11. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid
    #221997
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    DddA: S1330I, A1341V, N1342S, E1370K, T1380I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221997-plasmid-map-sequence-id-438639

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  15. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

    Plasmid
    #154829
    Purpose
    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    coEPO
    coEPO
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    Codon-optimized (Cricetulus griseus)
    Promoter
    100RPU.2
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154829-plasmid-map-sequence-id-308567

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  16. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  17. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid
    #169490
    Purpose
    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    Expression
    Mammalian
    Promoter
    PhCMV
    Available Since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    169490-plasmid-map-sequence-id-364218

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  20. shNTC

    Plasmid
    #73549
    Purpose
    Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).
    Depositor
    Gerardo Ferbeyre
    Article
    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d
    Insert
    shNTC
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    LTR
    Available Since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 10801 - 10820 of 13240 results