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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 34,543 results for: Sele

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Showing: 10801 - 10820 of 34543 results

  1. p-mCherry-C1-iC++_CA

    Plasmid
    #98168
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synthetic construct iC++_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98168-plasmid-map-sequence-id-182759

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  3. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pAraH6HATT

    Plasmid
    #63901
    Purpose
    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
    Depositor
    Carlos Barbas
    Publication
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Tags
    6xHis and HA tag
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    63901-plasmid-map-sequence-id-136457

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  11. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  14. TagBFP2-INPP4BC842A-CAAX

    Plasmid
    #116858
    Purpose
    inactive plasma membrane targetted PI(3,4)P2 phosphatase
    Depositor
    Gerry Hammond
    Publication
    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INPP4B (INPP4B Human)
    Tags
    HRAS(172-189) and TagBFP2
    Expression
    Mammalian
    Mutation
    catalytic cysteine 842 changed to alanine
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    116858-plasmid-map-sequence-id-224772

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  15. pEGFP-MYO10-HMM

    Plasmid
    #87256
    Purpose
    Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).
    Depositor
    Thomas Friedman
    Publication
    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYO10 (Myo10 Mouse)
    Expression
    Mammalian
    Mutation
    Myo10 is truncated to contain aa.1-941
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87256-plasmid-map-sequence-id-176080

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  16. GFP-DroshaS300/302A

    Plasmid
    #62530
    Purpose
    Serines 300 and 302 of Drosha are mutated to alanines
    Depositor
    Bharat Ramratnam
    Publication
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serines 300 and 302 to alanines
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid
    #55687
    Purpose
    expresses constitutively active human PKCi tagged with V5
    Depositor
    Pedro Salas
    Publication
    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    A120E
    Promoter
    CMV
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 10801 - 10820 of 34543 results