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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

Plasmid

#193349

Purpose

tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKS913.CB18.31

Plasmid

#69569

Purpose

TCR beta chain genomic transgenic construct for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

Insert

TCR beta (Tcrb Mouse)

Use

Mouse Targeting

Expression

Bacterial

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2

Plasmid

#229738

Purpose

Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-WT

Plasmid

#135997

Purpose

WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-EEA1

Plasmid

#174451

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAV U6+27

Plasmid

#25709

Purpose

for nuclear expression of small RNAs in mammalian cells

Depositor

David Engelke

Article

Good et al Gene Ther. 1997 Jan . 4(1):45-54.

Insert

U6 snRNP (LSM2 Human)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_2XMTS-MicroID2-FLAG

Plasmid

#188524

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

2XMTS-MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#133786

Purpose

Cre-dependent tdTomato fluorescent reporter for cell labeling

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

pAAV-EF1a-DIO-tdTomato-WPRE

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPC1 MOSPD3

Plasmid

#226405

Purpose

Expression of human MOSPD3 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

MOSPD3 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3C-GFP

Plasmid

#123110

Purpose

Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mTagBFP2

Plasmid

#122373

Purpose

Expresses mTagBFP2 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

mTagBFP2

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

Plasmid

#191157

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP2:Bdnf.s1:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP(1-10)

Plasmid

#70219

Purpose

Expresses GFP(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has Service

Cloning Grade DNA

Insert

GFP(1-10)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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