We narrowed to 21,861 results for: SPR

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pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

Plasmid

#176460

Purpose

Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV

Use

CRISPR

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSico_U6-Pan-PGC1a sgRNA

Plasmid

#165426

Purpose

Expression of gRNA against human Total PGC-1a variants

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against human Total PGC-1a variants (PPARGC1A Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-ACSL3-sfGFP(C) HDR template

Plasmid

#129413

Purpose

HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

ACSL3 HDR template (ACSL3 Human)

Use

CRISPR and TALEN; Endogenous tagging hdr template

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAG182 FGFR1 3'UTR mut

Plasmid

#12009

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N15 3'UTR mut (FGFR1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
AA183

Plasmid

#216025

Purpose

Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (H840A)_v2.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA028

Plasmid

#216008

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA060

Plasmid

#216017

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

nCas9 (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCK760

Plasmid

#192643

Purpose

Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23110-sfGFP

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23110

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0202

Plasmid

#185626

Purpose

MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH009_proD_Cas12c-sgRNA-GFP_CAM

Plasmid

#183070

Purpose

Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pQCi

Plasmid

#154345

Purpose

Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (wtAcrIIA4)

Plasmid

#128115

Purpose

Expresses SpyCas9 fused to wild-type AcrIIA4

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (wtAcrIIA4)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZBTB1

Plasmid

#128235

Purpose

Donor vector for 3' FLAG tag of human ZBTB1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_SIX4_iso1

Plasmid

#128238

Purpose

Donor vector for 3' FLAG tag of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-C

Plasmid

#113757

Purpose

Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR and Gateway Cloning

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pPN144

Plasmid

#91673

Purpose

Express sgRNA targeting human TBC1D5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TBC1D5 (TBC1D5 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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