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pT7-V5-SBP-C1-HsNanos1-F40A_X

Plasmid

#147957

Purpose

Mammalian Expression of HsNanos1-F40A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-F40A (NANOS1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos1-W43E_X

Plasmid

#147958

Purpose

Mammalian Expression of HsNanos1-W43E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-W43E (NANOS1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos1-Y46E_X

Plasmid

#147959

Purpose

Mammalian Expression of HsNanos1-Y46E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-Y46E (NANOS1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA

Plasmid

#164794

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG31TAG

Plasmid

#188996

Purpose

Express chimadanin with TAG at 28 and 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 and 31position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi31TAG

Plasmid

#188985

Purpose

Express chimadanin with TAG at 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 31 position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR

Plasmid

#178905

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Promoter

SV40 promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaA

Plasmid

#167810

Purpose

Expresses His6-tagged BhaA in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaB1

Plasmid

#167811

Purpose

Expresses His6-tagged BhaB1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaB7

Plasmid

#167813

Purpose

Expresses His6-tagged BhaB7 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB7

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-bhaC1

Plasmid

#167814

Purpose

Expresses BhaC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaC1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-E361Q-dsRNAres-V5His6_J

Plasmid

#146689

Purpose

Insect Expression of DmDCP2-E361Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmDCP2-E361Q-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164796

Purpose

pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK035

Plasmid

#180827

Purpose

T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmB

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK036

Plasmid

#180828

Purpose

T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmA

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-9-HSP-11

Plasmid

#176882

Purpose

MoClo Level 0 plasmid, contains HSP

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

HSP terminator

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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