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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,084 results for: nes

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Showing: 10861 - 10880 of 16084 results

  1. pPF3929

    Plasmid
    #254228
    Purpose
    LseCas13a expression plasmid including a CRISPR array for insertions of new spacers via BsmBI
    Depositor
    Peter Fineran
    Type
    Empty backbone
    Use
    CRISPR; Lsecas13a expression backbone for spacer …
    Promoter
    Lac promoter for LseCas13a and JS23119 promoter f…
    Available Since
    July 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    254228-plasmid-map-sequence-id-517549

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  4. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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  6. AA271

    Plasmid
    #216074
    Purpose
    Fragmid fragment: (CROPseq) guide expression; UMI-compatible
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216074-plasmid-map-sequence-id-431520

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  7. AA269

    Plasmid
    #216073
    Purpose
    Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
    Use
    Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216073-plasmid-map-sequence-id-431466

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  8. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  12. pKMDDV_Plasmid_1

    Plasmid
    #249037
    Purpose
    Plasmid 1 containing constitutively expressed dCas9 and RBP-AD along with inducible scRNA_RFP
    Depositor
    Domitilla Del Vecchio
    Article
    Aravind et al Cell Systems, 2026; 17
    Inserts
    dCas9
    MCP-SoxS(R93A/S101A)
    scRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    BBa_J23107, BBa_J23116, and PphlF
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    249037-plasmid-map-sequence-id-497730

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  13. pLV EF1a dCas9VP64 2A EGFP 2A BSD

    Plasmid
    #255458
    Purpose
    Expresses dCas9VP64, eGFP, and blasticidin
    Depositor
    Le Cong
    Insert
    dcas9-vp64
    Expression
    Mammalian
    Available Since
    May 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    255458-plasmid-map-sequence-id-510788

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  16. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  17. pCSD_SpCas9MT3-FKBP_SL

    Plasmid
    #107302
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107302-plasmid-map-sequence-id-204632

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Showing: 10861 - 10880 of 16084 results