We narrowed to 11,701 results for: phen
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HA-MBP-TEVpCS-ADGRE3 CTF-FLAG
Plasmid#217691PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-338Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTF-FLAG
Plasmid#217690PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-517Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-mCit-PA
Plasmid#113449PurposeProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsmCitrine-ProteinAExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-C601A
Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
TagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArC Long WT
Plasmid#59809PurposeAllows the integration of Myc ArC Long in the genome and Tet-inducible expression.DepositorInsertAurora C (AURKC Human)
TagsMycExpressionMammalianPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL C162F-pRc/CMV
Plasmid#22042DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 162 from Cysteine to …Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-S-PURO
Plasmid#58244PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistanceDepositorInsertsattR1-Cm(R)-CcdB-attR2
Puro(R)
UseLentiviral and RNAi; Gateway destination vectorExpressionMammalianPromoterSFFV and noAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-GW
Plasmid#113448PurposeProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsProteinA-mCitrineExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpPGIAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-short Cre-on G/W
Plasmid#86949PurposeGateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAVDepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL L158S-pRc/CMV
Plasmid#22041DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationpoint mutant converting aa 162 from Cysteine to P…Available SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-ccdB-6stop
Plasmid#203511PurposeGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-3x-uC-ccdB-6stop
Plasmid#203514PurposeGateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only
