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pcDNA3.1 NL-BCL2L1

Plasmid

#113458

Purpose

NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-600-1400

Plasmid

#79109

Purpose

Mammalian expression of GFP-KIF1B beta-600-1400

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
iChr2-Control-Mosaic (SO274)

Plasmid

#99751

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC403 LacZ

Plasmid

#111184

Purpose

Control inducible vector with LacZ (V5 tagged)

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

lacZ

Use

Lentiviral

Tags

V5

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC401 LacZ

Plasmid

#111183

Purpose

Control inducible vector with LacZ (V5 tagged)

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

lacZ

Use

Lentiviral

Tags

V5

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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VARP GFP-pLXIN

Plasmid

#62950

Purpose

full length human VARP with C-terminal EGFP tag cloned into pLXIN

Depositor

Paul Luzio

Article

Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

Insert

VARP (ANKRD27 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations of amino acids 422-428 to produc…

Promoter

LTR

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_7

Plasmid

#60293

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-rMERTK-HITI

Plasmid

#87119

Purpose

Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA

Use

AAV and CRISPR

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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iMb-Notch-Mosaic (IR99.40)

Plasmid

#99749

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 Luciferase

Plasmid

#117072

Purpose

single guide RNA targeting Luciferase

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

Luciferase

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_25

Plasmid

#60305

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_9

Plasmid

#60295

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_29

Plasmid

#60309

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFP36L2 enhancer (ZFP36L2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_10

Plasmid

#60259

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

RFX6 enhancer (RFX6 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_25

Plasmid

#60268

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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