We narrowed to 2,568 results for: CCS

1. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFL-SV40-GNB2 5'-UTR

   Plasmid

   #115355

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   GNB2 5'-UTR (GNB2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EZH2-TALEN_Right_No_FLAG

   Plasmid

   #79900

   Purpose

   Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Insert

   EZH2-TALEN_Right_No_FLAG (EZH2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAP3K7CL_pCSdest

   Plasmid

   #53808

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   MAP3K7CL (C21orf7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. YFP(1-158)-STOP

   Plasmid

   #66107

   Purpose

   This vector attaches YFP(1-158) to the C-terminus of a protein

   Depositor

   Catherine Berlot

   Article

   Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

   Insert

   YFP(1-158)STOP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Gln69 was changed to Met.

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY036_ATP1A1_G5

   Plasmid

   #86618

   Purpose

   Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRT/TO-p97-RG-mycStrep

   Plasmid

   #31840

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   R95G

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pET-22HT-MSI1(7-103)

   Plasmid

   #60357

   Purpose

   Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-103) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains amino acids 7-103 of Msi1

   Promoter

   T7

   Available since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET-22HT-MSI1(7-192)

   Plasmid

   #60245

   Purpose

   Express the RBD of mouse MSI1 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains aa7-192 of Msi1

   Promoter

   T7

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-22HT-MSI1(7-192)R61E

    Plasmid

    #60353

    Purpose

    R61E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R61E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

    Plasmid

    #233425

    Purpose

    IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CA-0216 CMVTO-hIL6Ra_M4-rens-hIL6-rens-IL6Rb

    Plasmid

    #231194

    Purpose

    Multi-cageIL-6

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    hIL6Ra_M4-rens-hIL6-rens-IL6Rb (IL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A228D, N230D, H280S, D281V

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CA-0251 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens

    Plasmid

    #231216

    Purpose

    myc-CleaveIL-10

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    c-myc-mono-hIL10 (R5A11M)-rens (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CA-0091 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra

    Plasmid

    #231191

    Purpose

    CageIL-10-monomer

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    mono-hIL10-R5A11M-rens-3GS-IL10Ra (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_RTN3E-mScarlet3

    Plasmid

    #216255

    Purpose

    mammalian expression vector for RTN3E-mScarlet3

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon 3 isoform e (RTN3 Human)
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_NPM2_cterm1

    Plasmid

    #222911

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_NPM2_cterm4

    Plasmid

    #222914

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_NPM2_cterm3

    Plasmid

    #222913

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

    Plasmid

    #222915

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_NPM2_cterm2

    Plasmid

    #222912

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge