We narrowed to 1,500 results for: RCA

1. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScaffold-H1 donor

   Plasmid

   #118152

   Purpose

   PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-T2A-HygR

   Plasmid

   #118153

   Purpose

   SpCas9 expression vector

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   hSpCas9-T2A-HygR
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BAP1-wt (siRNA resistant)

   Plasmid

   #108439

   Purpose

   Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

   Promoter

   CMV

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BAP1-C91A (siRNA resistant)

   Plasmid

   #108438

   Purpose

   Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C91A: c.271T>G, c.272G>C; others: c.1641C&g…

   Promoter

   CMV

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiSAMv2 ZFAND3-guide4

   Plasmid

   #125516

   Purpose

   CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

   Plasmid

   #222625

   Purpose

   Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of the residues 561-625 (ΔNES)

   Promoter

   EF-1-alpha promoter

   Available Since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. lentiSAMv2 ZFAND3-guide2

   Plasmid

   #125514

   Purpose

   CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiSAMv2 ZFAND3-guide1

   Plasmid

   #125513

   Purpose

   CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiSAMv2 ZFAND3-guide3

    Plasmid

    #125515

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118155

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiSAMv2 OPTN TSS-guide4

    Plasmid

    #118199

    Purpose

    CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiSAMv2 EGFP-guide1

    Plasmid

    #118166

    Purpose

    CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiSAMv2 ZBED3-TSS-guide1

    Plasmid

    #125506

    Purpose

    CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiSAMv2 CRY2-TSS-guide3

    Plasmid

    #125491

    Purpose

    CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiSAMv2 CRY2-TSS-guide2

    Plasmid

    #125490

    Purpose

    CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiSAMv2 TCF7L2 TSS-guide1

    Plasmid

    #118184

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiSAMv2 TCF7L2 TSS-guide4

    Plasmid

    #118187

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiSAMv2 TCF7L2 TSS-guide3

    Plasmid

    #118186

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 EGFP-guide2

    Plasmid

    #118167

    Purpose

    CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge