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We narrowed to 14,999 results for: flag

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Showing: 1081 - 1100 of 14999 results

  3. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92114-plasmid-map-sequence-id-197615

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  8. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid
    #107307
    Purpose
    Encoding an SPH activation system
    Depositor
    Hui Yang
    Publication
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    Expression
    Mammalian
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107307-plasmid-map-sequence-id-457328

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  11. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70708-plasmid-map-sequence-id-364684

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  14. pX330-Cetn1/1

    Plasmid
    #50718
    Purpose
    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Publication
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50718-plasmid-map-sequence-id-75953

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  18. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64709-plasmid-map-sequence-id-322473

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  20. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Bradley Bernstein
    Publication
    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170186-plasmid-map-sequence-id-336799

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Showing: 1081 - 1100 of 14999 results