We narrowed to 19,230 results for: REV;

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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231346

Purpose

Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Promoter

CamKIIa

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-egfp-U3-Osp.gRNA

Plasmid

#166034

Purpose

Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hSIRT3_H248Y_HA

Plasmid

#24492

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)

Tags

HA

Expression

Mammalian

Mutation

H248Y

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP-B19G

Plasmid

#52473

Purpose

Expresses splitTVA-EGFP-B19G

Depositor

Ian Wickersham

Article

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Has Service

AAV1

Insert

TVA-P2A-EGFP-P2A-B19G

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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p2,0_3M5M_luciferase

Plasmid

#135475

Purpose

Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, luciferase reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin-TEV-His6

Plasmid

#178067

Purpose

Insect cell expression of gamma-tubulin-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin (TUBG1 Human)

Tags

TEV-His6

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTW040

Plasmid

#64769

Purpose

For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH3

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pX330 _CRISPIE donor B8

Plasmid

#172849

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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