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V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-talin1 rod

Plasmid

#32855

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin 1 Rod aa 434-2541 (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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pMycMyt1-7zf/IRES-Red

Plasmid

#22652

Depositor

Lynn Hudson

Article

Nielsen et al Mol Cell Neurosci. 2004 Jan . 25(1):111-

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-T4uvsW

Plasmid

#228201

Purpose

Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

Depositor

Stephen Benkovic

Article

Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

Insert

uvsW

Tags

hexahistidine and 10 amino acid linker

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-eDHFR

Plasmid

#176201

Purpose

Negative control of targeted optogenetic manipulation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-eDHFR

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Sapphire

Plasmid

#96950

Purpose

Retroviral plasmid containing Sapphire for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Sapphire fluorescent protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
2 gRNA concatmer

Plasmid

#84879

Purpose

Empty concatmer vector in which 2 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pDB007(+)

Plasmid

#99147

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)

Expression

Bacterial

Promoter

PT7 and PproK

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMyCA-mCherry

Plasmid

#84272

Purpose

mCherry fluorescence protein expression using M. smegmatis

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Insert

mCherry

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSL5200 (pDonor_ISGst3)

Plasmid

#210875

Purpose

Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

ISGst3

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDB038

Plasmid

#99210

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*), luxAB; PproK pylT

Expression

Bacterial

Mutation

amber codons at position 29 gIII

Promoter

Ppsp and PproK

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Bacterial

Promoter

lacI

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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PE-GsI-IIC-RT

Plasmid

#181808

Purpose

PE-GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-GsI-IIC-RT

Use

CRISPR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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SRpHi4

Plasmid

#102544

Purpose

Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

EYFP-EGF

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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