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pBzCas13b-gRNA-NT

Plasmid

#164861

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Epsin1-ECFP

Plasmid

#21066

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin 1 (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pCIP

Plasmid

#79009

Purpose

For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR

Plasmid

#157794

Purpose

Expression of sfGFP-TetR fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE12

Plasmid

#90347

Purpose

FXR - Farnesoid X receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

FXR

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Epsin1 deltaUIM-ECFP

Plasmid

#20973

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin1 deltaUIM (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

June 15, 2009

Availability

Academic Institutions and Nonprofits only
GFP-Rnd1

Plasmid

#23227

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd1 (RND1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

Plasmid

#58435

Purpose

Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)8-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGateway 3XFlag Prickle2 flag tagged

Plasmid

#24645

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Prickle 2 (PRICKLE2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Article

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-SnoopTagJr-MBP

Plasmid

#105628

Purpose

Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SnoopTagJr-MBP

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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RAB14 shRNA

Plasmid

#61496

Purpose

to knockdown endogenous RAB14 expression levels

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński, Joshua Sanes

Article

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Insert

LL5b (Phldb2 Mouse)

Tags

TagRFP

Expression

Mammalian

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only