We narrowed to 19,702 results for: CAL

Showing: 11021 - 11040 of 19702 results

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KG#901

Plasmid

#110936

Purpose

Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

mig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.NgCAM-Halo

Plasmid

#162719

Purpose

Mammalian expression of chicken NgCam

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

NgCam (L1CAM Chicken)

Tags

Halo

Expression

Mammalian

Mutation

E137K, K138E, and K642E

Promoter

Chicken Beta actin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR0B1_Hormone-recep

Plasmid

#109835

Purpose

Protein expression and purification of NR0B1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR0B1_Hormone-recep (NR0B1 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HLA-DRB1_MHC-II-beta

Plasmid

#110021

Purpose

Protein expression and purification of HLA-DRB1_MHC-II-beta

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HLA-DRB1_MHC-II-beta (HLA-DRB1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR5A1_Hormone-recep

Plasmid

#109792

Purpose

Protein expression and purification of NR5A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR5A1_Hormone-recep (NR5A1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Nup124-GFP-MTn

Plasmid

#154365

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Expression

Yeast

Mutation

See depositor comments below

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab1ab

Plasmid

#109606

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab1ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab1ab (rab1ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAb

Plasmid

#109579

Purpose

Multisite gateway vector for 3' tagging with RhoAb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAb (rhoab Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKM296

Plasmid

#134180

Purpose

Mammalian expression of CENP-C and KNL2 CDK SA

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPshRNA1

Plasmid

#126611

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-1 (CNP Human)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-V5-Trpt1

Plasmid

#126607

Purpose

Expresses N-terminal V5-tagged mouse Trpt1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA 2' phosphotransferase 1 (Trpt1 Mouse)

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-HipO

Plasmid

#114599

Purpose

Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

HipO

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

OR2-OR1-Pr

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-N273K

Plasmid

#115617

Purpose

Expresses human Parkin N273K in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

N273K

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_PLRKT

Plasmid

#111673

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

PLRKT (PLGRKT Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DST-1/1

Plasmid

#91288

Purpose

Protein expression and purification of human SH3 domain construct DST-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DST-1/1 (DST Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-HPII TAG415

Plasmid

#105847

Purpose

expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

hydroperoxidase II catalase

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 415

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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