We narrowed to 67,456 results for: cell;

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pFX-iRFP-Rab11

Plasmid

#174462

Purpose

Express iRFP with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf1(Q71L)-HA

Plasmid

#79410

Purpose

Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

Plasmid

#191144

Purpose

Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:PYL1:nls:VPR

Plasmid

#135988

Purpose

Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

PYL1 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

Plasmid

#67294

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-ABEmax

Plasmid

#164415

Purpose

EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

ABEmax

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-7

Plasmid

#126055

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-S149A

Plasmid

#135998

Purpose

S149A G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf1(T31N)-HA

Plasmid

#79411

Purpose

Expresses C-terminally HA-tagged Arf1(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-FlpOn-CreOff-TdTomato

Plasmid

#176275

Purpose

Viral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tdTomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A382T

Plasmid

#133756

Purpose

Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Mutation

Changed alanine 382 to threonine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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cASPG1-mRFP

Plasmid

#181962

Purpose

ASPG1 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ASPG1 (ASPG1 Mustard Weed)

Tags

mRFP

Expression

Plant

Promoter

TBA2 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag-TMCC1

Plasmid

#121045

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

3x-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS7

Plasmid

#218715

Purpose

Expresses N-terminally EGFP-tagged BBS7 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS7 (BBS7 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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