We narrowed to 90,070 results for: Mal

1. pRK5-LYCHOS (GPR155) WT-HA

   Plasmid

   #199660

   Purpose

   Transient expression of LYCHOS (GPR155) WT

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pODN-mNG-ECT2

   Plasmid

   #183835

   Purpose

   Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

   Plasmid

   #114089

   Purpose

   CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized FnCas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139088

   Purpose

   Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   A262T, R324L,S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-UBC-S2-RAB_EKARev

   Plasmid

   #160726

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-RAB_EKARev
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3_ATP10B

   Plasmid

   #203695

   Purpose

   expresses ATP10B

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LoxP1-His-H2B-Cherry-2A (SO290)

   Plasmid

   #99616

   Purpose

   To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   6xHis-H2B-Cherry
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139091

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nAChR beta2 CFP

    Plasmid

    #15106

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-CFP-M (Chrnb2 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCCL-PGK-SPdCas9-BFP-DNMT3B

    Plasmid

    #66820

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
    

    Tags

    dSpCas9-BFP-DNMT3B

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEX-CFP-STIM1-deltaK

    Plasmid

    #18860

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Stop codon at amino acid position 671 of STIM1. C…

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-shMBNL2-3712

    Plasmid

    #115461

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL2-3712 (MBNL2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-shMBNL2-1082

    Plasmid

    #115462

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL2-1082 (MBNL2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-shMBNL1-3964

    Plasmid

    #115459

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL1-3964 (MBNL1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-AID'-seBE4max-IRES

    Plasmid

    #174697

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    AID'-seBE4max-IRES (AICDA Human)
    

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    UGI-E11K

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-BRCA1 3'UTR

    Plasmid

    #29477

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    BRCA1 3'UTR (BRCA1 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid

    #127483

    Purpose

    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    One nucleotide inserted between lambda CI and LGF…

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide-Neo-NCOA7g1 (BB34)

    Plasmid

    #139452

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge