We narrowed to 18,398 results for: Mut

1. pKM197

   Plasmid

   #132665

   Purpose

   pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

   Depositor

   Joseph Sorg

   Article

   Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

   Insert

   Upstream & Downstream pyrE deletion region
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCZGY2850

   Plasmid

   #87758

   Purpose

   Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pcol-19-PH-miniSOG(Q103L)
   

   Use

   Tags

   PH

   Expression

   Worm

   Mutation

   Q103L

   Promoter

   Pcol-19

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA HA FAK Y407F

   Plasmid

   #50504

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y407F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pESC-URA-GFP-Ubc9ts

   Plasmid

   #20369

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y68L This mutation renders Ubc9 temperature-sens…

   Promoter

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pYPQ230-D156R (ttLbCas12a)

   Plasmid

   #195366

   Purpose

   A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   LbCas12a-D156R (ttLbCas12a)
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(-) FLIPQTV3.0 8m

   Plasmid

   #63732

   Purpose

   FLIPQTV3.0 glutamine sensor with R75MY86A mutations

   Depositor

   Sakiko Okumoto

   Article

   Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

   Insert

   FLIPQTV3.0 sensor, 8mM affinity
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. hTRAF2pLPCX-HA-Flag/P874

   Plasmid

   #20229

   Depositor

   Martin Dorf

   Article

   Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

   Insert

   TNF receptor-associated factor 2 (TRAF2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   unmutated NM_021138.3. L replaces M at beginning…

   Promoter

   Available Since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-RFP-p2a-postASAP

   Plasmid

   #178793

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with RFP

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and TagRFP
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Paxillin

   Plasmid

   #186142

   Purpose

   For expresseion of recombinant paxillin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Paxillin C1997A (silent mutation for cloning)

   Promoter

   Lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

    Plasmid

    #127404

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJH726_pJH_LldR_PlldR-sfGFP.15TAG

    Plasmid

    #163730

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.15TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp with first 15 leucine codons mutated to amber

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. R4227X-F

    Plasmid

    #21371

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG (following D4226)

    Expression

    Mammalian

    Mutation

    Changed R4227 to stop codon

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-Casp7 C186A-Flag

    Plasmid

    #11816

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C186A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

15. YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)

    Plasmid

    #233383

    Purpose

    ER-retained catalytically inactive ADAR2dd (E396A mutation)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    TM-ADAR2dd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E396A

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBFRE-(mt)-luc

    Plasmid

    #26896

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated CBF1 sites (see note)

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET51b(+)_MBP-nLucCP-67/68

    Plasmid

    #113922

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-67/68
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Promoter

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PTEN-C124S

    Plasmid

    #28300

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phosphatase Inactive Mutant Cys124Ser

    Promoter

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only