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pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

Plasmid

#101213

Purpose

Bacterial expression plasmid for SpCas9 + K855A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K855A

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-224N

Plasmid

#31634

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

1st 224 amino acids of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pJK597

Plasmid

#71121

Purpose

Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Mutation

contains bp# 1004-1638 of BC006834.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Chn2 in situ probe

Plasmid

#45625

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Chn2 in situ probe (Chn2 Mouse)

Use

In situ

Mutation

fragment contains bp# 2212-2619 of BC051139.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Inhba

Plasmid

#45868

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Inhba in situ probe (Inhba Mouse)

Use

In situ

Mutation

nt 428-933 from Inhba mRNA (NM_008380.1)

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-Cas9-T2A-mNeonGreen-2-bc6

Plasmid

#250524

Purpose

Expresses Cas9 and mNeonGreen. Barcode is added to the vector.

Depositor

James Eshleman

Article

Teh et al bioRxiv [Preprint]. 2023 Apr 5:2023.04.0

Insert

M13F-AATTGGCC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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C4orf19-P1

Plasmid

#236322

Purpose

Study the role and mechanism of the 3' UTR in regulating C4orf19-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

C4orf19-P1 3'UTR (C4orf19 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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C4orf19-P2

Plasmid

#236323

Purpose

Study the role and mechanism of the 3' UTR in regulating C4orf19-P2 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

C4orf19-P2 3'UTR (C4orf19 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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DVL3-P2

Plasmid

#236026

Purpose

Study the role and mechanism of the 3' UTR in regulating DVL3-P2 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

DVL3-P2 3'UTR (DVL3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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ITGB6-P1

Plasmid

#236031

Purpose

Study the role and mechanism of the 3' UTR in regulating ITGB6-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

ITGB6-P1 3'UTR (ITGB6 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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ITGB6-P3

Plasmid

#236032

Purpose

Study the role and mechanism of the 3' UTR in regulating ITGB6-P3 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

ITGB6-P3 3'UTR (ITGB6 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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AKIRIN1-P1

Plasmid

#236018

Purpose

Study the role and mechanism of the 3' UTR in regulating AKIRIN1-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

AKIRIN1-P1 3'UTR (AKIRIN1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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AKIRIN1-P2

Plasmid

#236019

Purpose

Study the role and mechanism of the 3' UTR in regulating AKIRIN1-P2 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

AKIRIN1-P2 3'UTR (AKIRIN1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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ALDH5A1-P1

Plasmid

#236020

Purpose

Study the role and mechanism of the 3' UTR in regulating ALDH5A1-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

ALDH5A1-P1 3'UTR (ALDH5A1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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ALDH5A1-P2

Plasmid

#236021

Purpose

Study the role and mechanism of the 3' UTR in regulating ALDH5A1-P2 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

ALDH5A1-P2 3'UTR (ALDH5A1 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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DVL3-P1

Plasmid

#236025

Purpose

Study the role and mechanism of the 3' UTR in regulating DVL3-P1 gene function.

Depositor

Angela Ting

Article

Le et al Cell Rep. 2026 Jan 13;45(1):116839. doi:

Insert

DVL3-P1 3'UTR (DVL3 Human)

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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