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pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
miniSOG-Alpha-V-Integrin-25

Plasmid

#57763

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-V Integrin (ITGAV Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
AAV-hSyn-Lyn-Y-GECO1

Plasmid

#58200

Purpose

Express the membrane targeted Y-GECO1 in neurons

Depositor

Robert Campbell

Article

Zhao et al Integr Biol (Camb). 2014 May 19.

Insert

Y-GECO1.0

Use

AAV

Promoter

hSyn

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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TIRAP-TAP_IRES_GFP

Plasmid

#52740

Purpose

Retroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFP

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

3x HA, 3x TEV, and BirA target site

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pBMN-flag beta-catenin K19R/K49R

Plasmid

#44751

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R/K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMM2-hPMCA2x/b

Plasmid

#47582

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b (ATP2B2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C0-GFP

Plasmid

#46040

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2006 Aug 25;281(34):24662-7

Insert

nonmuscle myosin heavy chain II-C, isoform C0 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pAcGP67A-rCL1-HormR-GAIN

Plasmid

#37847

Depositor

Axel Brunger

Article

Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

Insert

CL1 (Adgrl1 Rat)

Use

Baculovirus transfer vectors

Tags

8xHIS

Mutation

HormR and GAIN domains only (aa460–849

Promoter

Polyhedrin

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 ERbeta(1-255)

Plasmid

#35564

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Mutation

residues 1-255

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Gateway Cloning

Tags

FLAG and mCherry-P2A

Mutation

D100A

Promoter

short TetO, miniCMV

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q80-C14A

Plasmid

#22121

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pIS1 Cyclin D2 short UTR

Plasmid

#21644

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 short UTR (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMIG small T C97S E102Q

Plasmid

#9060

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

C97S, E102Q

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

Plasmid

#229787

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; ZF10-NS3-p65

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)

Plasmid

#214846

Purpose

Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

ChR2(H134R)-EYFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-mCherry

Plasmid

#220351

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-enCas12a-2xNLS

Plasmid

#209021

Purpose

Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

enCas12a-2xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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