We narrowed to 25,304 results for: crispr

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1053B

Plasmid

#184007

Purpose

Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

Depositor

Omar Akbari

Publication

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAhh1 - U6c:sgRNAhh2

Expression

Insect

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-egfp-U3-UL8

Plasmid

#166694

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Depositor

Yujia Cai

Publication

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)

Use

Lentiviral

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.5

Plasmid

#158407

Purpose

Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162337

Purpose

Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-GSX2-tTA-BleoR

Plasmid

#161748

Purpose

Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Publication

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

Advanced tTA

Use

CRISPR

Expression

Mammalian

Promoter

endogenous GSX2 promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Publication

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Publication

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Expression

Bacterial

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141C2.0

Plasmid

#99905

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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B270 + SPRTN sgSTOP

Plasmid

#100718

Purpose

B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

Depositor

Alberto Ciccia

Publication

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgSTOP targeting SPRTN (cloned using BbsI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSL3927 (pDonor(plasmid)_Pse)

Plasmid

#200905

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (pTarget NGS)

Expression

Mammalian

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188765

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pmiCas9-msa

Plasmid

#164833

Purpose

miCas9-msa fusion protein expressing plasmid

Depositor

Eugene Chen

Publication

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miCas9-msa

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

contains mSA sequence range is 5106-5447 and Brex…

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMP1183

Plasmid

#119254

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Publication

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Expression

Mammalian

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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