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175,655 results

Showing: 2751 - 2800 of 175655 results

  2. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA
    Available Since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  6. pCWXPGR-pTF-Kir2.1

    Plasmid
    #114284
    Purpose
    TET-inducible expression of the Kir2.1 protein
    Depositor
    Patrick Salmon
    Insert
    Kir2.1 (KCNJ2 Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114284-plasmid-map-sequence-id-218907

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  8. pAAV-EF1a-Flp-DOG-NW (AAV1)

    Viral Prep
    #75469-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flp-DOG-NW (#75469). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flp-DOG-NW plasmid DNA. EF1a-driven expression of Flp recombinase dependent on GFP (Flp-DOG); to be coinjected with GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:
    Promoter
    EF-1a
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-FLEX-tdTomato (AAV1)

    Viral Prep
    #28306-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  24. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134908-plasmid-map-sequence-id-342556

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  25. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  26. pAAV-hSyn-EGFP (AAV5)

    Viral Prep
    #50465-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. Yes-EGFP

    Plasmid
    #110497
    Purpose
    Fluorescent Yes
    Depositor
    Bernardo Mainou
    Insert
    Yes (YES1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    110497-plasmid-map-sequence-id-210495

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  30. pLenti CMV GFP Hygro (656-4) (Lentiviral Prep)

    Viral Prep
    #17446-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from pLenti CMV GFP Hygro (656-4) (#17446). In addition to the viral particles, you will also receive purified pLenti CMV GFP Hygro (656-4) plasmid DNA. Lentiviral particles carrying the GFP and hygromycin resistance.
    Depositor
    Eric Campeau, Paul Kaufman
    Article
    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only

  31. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)

    Viral Prep
    #75470-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Connie Cepko
    Article
    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:
    Promoter
    CAG
    Tags
    mCherry
    Available Since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  33. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV PHP.eB)

    Viral Prep
    #44361-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

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  38. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  39. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep
    #105551-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    GFP
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.S)

    Viral Prep
    #59462-PHP.S
    Purpose
    Ready-to-use AAV PHP.S particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-Ef1a-fDIO mCherry (AAV8)

    Viral Prep
    #114471-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  47. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

    Viral Prep
    #51085-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available Since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 2751 - 2800 of 175655 results