We narrowed to 24,746 results for: CHI
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Plasmid#223745PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only
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pPBbsr2-EREG-ScNeo
Plasmid#209909PurposeTo monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-ScNeo (EREG Human)
TagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP703-eDHFR(69K6)-cRaf
Plasmid#209919PurposeMammalian expression of cRaf fused to miRFP703-eDHFR(69K6)DepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-miRFP703-eDHFR(69K6)-cRaf
Plasmid#209920PurposeMammalian expression of cRaf fused to miRFP703-eDHFR(69K6)DepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-miRFP703-eDHFR(69K6)-cRaf
Plasmid#209921PurposeMammalian expression of cRaf fused to miRFP703-eDHFR(69K6)DepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Human)
ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-HBEGF-ScNeo
Plasmid#209903PurposeTo monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-HBEGF-ScNeo
Plasmid#209907PurposeTo monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-W
Plasmid#200487PurposeLentiviral vector expressing gRNA targeting human CDK6-E1DepositorInsertCDK6-E1(6) (CDK6 Human)
UseLentiviralAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ARID2(13))-PGKpuro2ABFP-W
Plasmid#200471PurposeLentiviral vector expressing gRNA targeting human ARID2DepositorInsertARID2(13) (ARID2 Human)
UseLentiviralAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BRD9(13))-PGKpuro2ABFP-W
Plasmid#200473PurposeLentiviral vector expressing gRNA targeting human BRD9DepositorInsertBRD9(13) (BRD9 Human)
UseLentiviralAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-W
Plasmid#200493PurposeLentiviral vector expressing gRNA targeting human RHOADepositorInsertRHOA(15) (RHOA Human)
UseLentiviralAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MYC_2)-PGKpuroBFP-W
Plasmid#211970PurposeExpress gRNA against MYC with puro and BFPDepositorInsertsgRNA targeting MYC (MYC Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(MYC_7)-PGKpuroBFP-W
Plasmid#211971PurposeExpress gRNA against MYC with puro and BFPDepositorInsertsgRNA targeting MYC (MYC Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NANOG_g1)-PGKpuroBFP-W
Plasmid#211974PurposeExpress gRNA against NANOG with puro and BFPDepositorInsertsgRNA targeting NANOG (NANOG Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NANOG_g2)-PGKpuroBFP-W
Plasmid#211975PurposeExpress gRNA against NANOG with puro and BFPDepositorInsertsgRNA targeting NANOG (NANOG Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ETS2_5-5)-PGKpuroBFP-W
Plasmid#211959PurposeExpress gRNA against ETS2 with puro and BFPDepositorInsertsgRNA targeting ETS2 (ETS2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(FOXH1_5-2)-PGKpuroBFP-W
Plasmid#211962PurposeExpress gRNA against FOXH1 with puro and BFPDepositorInsertsgRNA targeting FOXH1 (FOXH1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(FOXH1_5-4)-PGKpuroBFP-W
Plasmid#211963PurposeExpress gRNA against FOXH1 with puro and BFPDepositorInsertsgRNA targeting FOXH1 (FOXH1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ID1_5-2)-PGKpuroBFP-W
Plasmid#211964PurposeExpress gRNA against ID1 with puro and BFPDepositorInsertsgRNA targeting ID1 (ID1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ID1_5-5)-PGKpuroBFP-W
Plasmid#211965PurposeExpress gRNA against ID1 with puro and BFPDepositorInsertsgRNA targeting ID1 (ID1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ETS2_5-2)-PGKpuroBFP-W
Plasmid#211958PurposeExpress gRNA against ETS2 with puro and BFPDepositorInsertsgRNA targeting ETS2 (ETS2 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ETS1(12))-PGKpuro2ABFP-W
Plasmid#208550PurposeLentiviral vector expressing gRNA targeting human ETS1DepositorInsertETS1(12) (ETS1 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ETS1(11))-PGKpuro2ABFP-W
Plasmid#208549PurposeLentiviral vector expressing gRNA targeting human ETS1DepositorInsertETS1(11) (ETS1 Human)
UseLentiviralAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX1(13))-PGKpuro2ABFP-W
Plasmid#200501PurposeLentiviral vector expressing gRNA targeting human RUNX1DepositorInsertRUNX1(13) (RUNX1 Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX1(11))-PGKpuro2ABFP-W
Plasmid#200500PurposeLentiviral vector expressing gRNA targeting human RUNX1DepositorInsertRUNX1(11) (RUNX1 Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RHOA(12))-PGKpuro2ABFP-W
Plasmid#200492PurposeLentiviral vector expressing gRNA targeting human RHOADepositorInsertRHOA(12) (RHOA Human)
UseLentiviralAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CDK6-E2(12))-PGKpuro2ABFP-W
Plasmid#200488PurposeLentiviral vector expressing gRNA targeting human CDK6-E2DepositorInsertCDK6-E2(12) (CDK6 Human)
UseLentiviralAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CDK6-E1(1))-PGKpuro2ABFP-W
Plasmid#200486PurposeLentiviral vector expressing gRNA targeting human CDK6-E1DepositorInsertCDK6-E1(1) (CDK6 Human)
UseLentiviralAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(CDK6-E2(15))-PGKpuro2ABFP-W
Plasmid#200489PurposeLentiviral vector expressing gRNA targeting human CDK6-E2DepositorInsertCDK6-E2(15) (CDK6 Human)
UseLentiviralAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BRD9(91))-PGKpuro2ABFP-W
Plasmid#200474PurposeLentiviral vector expressing gRNA targeting human BRD9DepositorInsertBRD9(91) (BRD9 Human)
UseLentiviralAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ARID2(14))-PGKpuro2ABFP-W
Plasmid#200472PurposeLentiviral vector expressing gRNA targeting human ARID2DepositorInsertARID2(14) (ARID2 Human)
UseLentiviralAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ZC3H12D_PromoterMUT
Plasmid#153067PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE4040 binding sites.…PromoterNoneAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-6)
Plasmid#89730PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
ExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-12)
Plasmid#89731PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.DepositorInsertCRISPR array
ExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-18)
Plasmid#89732PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.DepositorInsertCRISPR array
ExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-3)
Plasmid#89729PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.DepositorInsertCRISPR array
ExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone
Plasmid#216154PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic exon and C-terminal FLAG
ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone
Plasmid#216153PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-mCherryBCL11B-W
Plasmid#208758PurposeLentiviral vector expressing mCherry fused with BCL11B cDNADepositorInsertBCL11B (BCL11B Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APP-mGFP
Plasmid#196704PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmCherry and mEGFPExpressionMammalianAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP-APP
Plasmid#196706PurposeExpresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsAP, Avitag and HAExpressionMammalianAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APP-mGFP
Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmEGFP and mtagBFP2ExpressionMammalianPromoterCMVAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
ExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV
Plasmid#216162PurposeExpresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertCre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
UseCre/LoxExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone
Plasmid#216155PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APPP1-mGFP
Plasmid#196695PurposeAs mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmEGFP and mtagBFP2ExpressionMammalianMutationLys to Val substitution at position 612PromoterCMVAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-mCherry-Halo-YAP1
Plasmid#128336PurposeRetrovirus construct to express mCherry-Halo-YAP1DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only