We narrowed to 10,438 results for: Ott
-
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
-
pCRISPR3_BC1
Plasmid#191856PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCL8
Plasmid#176184PurposegRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1tDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCL8_28bp
Plasmid#176185Purposeplasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1tDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCL9
Plasmid#176186Purpose2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1tDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI1up
Plasmid#176166Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1DepositorInsert5' homology for integration site XI-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI1dw
Plasmid#176167Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1DepositorInsert3' homology for integration site XI-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI2up
Plasmid#176168Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2DepositorInsert5' homology for integration site XI-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI2dw
Plasmid#176169Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2DepositorInsert3' homology for integration site XI-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XII2up
Plasmid#176174Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2DepositorInsert5' homology for integration site XII-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XII2dw
Plasmid#176175Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2DepositorInsert3' homology for integration site XII-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-X4up
Plasmid#176164Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4DepositorInsert5' homology for integration site X-4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-X4dw
Plasmid#176165Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4DepositorInsert3' homology for integration site X-4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG-altTAG
Plasmid#158146PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BXG
Plasmid#158145PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment (w/ TAG codon)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-BYG
Plasmid#158144PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertBFP-GFP fragment (wild-type)
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6
Plasmid#158142PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertFAPB2.3.6
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG
Plasmid#130678PurposeExpresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD409_F489delPromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG
Plasmid#130680PurposeExpresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-36.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD1523_G1960delPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG
Plasmid#130677PurposeExpresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-46.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationM1961_E1992delPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130691PurposeExpresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAG and 3xHAExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG
Plasmid#130689PurposeExpresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, M1961_E1992delPromoterCMVAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG
Plasmid#130686PurposeExpresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_G1960delPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG
Plasmid#130690PurposeExpresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, G1720_Y1846delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG
Plasmid#130685PurposeExpresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.41-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_Q1684delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130682PurposeExpresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG
Plasmid#130687PurposeExpresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_E1992delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab18 WT
Plasmid#58453Purposeexpression of drosophila YFP Rab18DepositorInsertRab18 (Rab18 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-GFP-Patronin CC 534–868
Plasmid#59054Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab19 WT
Plasmid#58454Purposeexpression of drosophila YFP Rab19DepositorInsertRab19 (Rab19 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab8 WT
Plasmid#58450Purposeexpression of drosophila YFP Rab8DepositorInsertRab8 (Rab8 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab8 WT
Plasmid#58449Purposeexpression of drosophila td tomato Rab8DepositorInsertRab8 (Rab8 Fly)
UseTagstd TomatoExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST myc Rab35 CA
Plasmid#58382Purposeexpression of drosophila myc-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsmycExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab35 CA
Plasmid#58381Purposeexpression of drosophila YFP-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab26 CA
Plasmid#53483DepositorInsertRab26 (Rab26 Fly)
UseTagsYFPExpressionInsectMutationQ250LPromoterGAL4Available sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab21 CA
Plasmid#37725DepositorInsertRab21 (Rab21 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ73L (constitutive active)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX4 CA
Plasmid#37776DepositorInsertRabX4 (RabX4 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab26 DN
Plasmid#37730DepositorInsertRab26 (Rab26 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT204N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab26 WT
Plasmid#37729DepositorInsertRab26 (Rab26 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab21 DN
Plasmid#37724DepositorInsertRab21 (Rab21 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT27N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab19 CA
Plasmid#37722DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ80L (constitutive active)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab19 DN
Plasmid#37721DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT35N (dominant negative)PromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab19 WT
Plasmid#37720DepositorInsertRab19 (Rab19 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX1 WT
Plasmid#37750DepositorInsertRabX1 (chrw Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX1 DN
Plasmid#37751DepositorInsertRabX1 (chrw Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT19N (dominant negative)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP RabX1 CA
Plasmid#37752DepositorInsertRabX1 (chrw Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ66L (constitutive active)PromoterGAL4Available sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab2 CA
Plasmid#37686DepositorInsertRab2 (Rab2 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab2 DN
Plasmid#37685DepositorInsertRab2 (Rab2 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-PGK-EiP
Plasmid#21312PurposeControl Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistanceDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only