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Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLKO.1-puro-shNT
Plasmid#109012Purposeshort-hairpin knockdown of targeted geneDepositorInsertNon-targeting shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPM47 (UPR-RFP CEN/ARS URA3)
Plasmid#20132DepositorInsertUPR-RFP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cyto_K0
Plasmid#133864PurposeExpression of cytoplasmic Ribosome non-stalling reporterDepositorInsertCyto_K0
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
luxB:Venus+luxA/pRSETB Duet
Plasmid#197582PurposeBRET lux luciferase with Venus for bacterial expressionDepositorInsertLuxA LuxBVenus
UseE.coli expressionTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-jRCaMP1b
Plasmid#233207PurposeRetroviral expression of jRCaMP1b calcium reporterDepositorInsertjRCaMP1b
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
luxA+luxB/pRSETB Duet
Plasmid#197581PurposeExpressing luxA and luxB under dual T7 promoterDepositorInsertLuxA LuxB
UseTagsT7 tag and 6xHisExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-ExRai-PKNAR
Plasmid#226633PurposePlasma membrane-targeted ExRai-PKNARDepositorInsertPM-ExRai-PKNAR
UseTagsExpressionMammalianMutationPlasma membrane targeting N-terminal 11 amino aci…PromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER_K0
Plasmid#133863PurposeExpression of ER-targeted Ribosome non-stalling reporterDepositorInsertER_K0
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJDC182
Plasmid#60854PurposeExpresses firefly luciferaseDepositorInsertFirefly luciferase (optimized for Mycobacteria)
UseTagsExpressionBacterialMutationcodon optimized for MycobacteriaPromoterhsp60Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
GW1-ARSeNL
Plasmid#207873PurposeMammalian expression of the BRET ATP sensorDepositorInsertARSeNL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mRuby-Sec23A
Plasmid#36158DepositorInsertSec23A (SEC23A Human)
UseLentiviralTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Blast-rLAMP1-eGFP
Plasmid#109028Purposestable lentiviral expression of cDNADepositorInsertrLAMP1
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
5HT6-GCaMP5G
Plasmid#48340PurposeFluorescent reporter for Ca2+ inside primary ciliaDepositorInsertGCaMP5G
UseTags5HT6ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
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EF.hICN1
Plasmid#113715PurposeConstitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.DepositorInsertNone
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B CPM2
Plasmid#121163PurposeMammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)DepositorInsertZFP36L1 (TIS11B) charge pattern mutant 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCM-HA-Gas1
Plasmid#38313DepositorInsertGas1 (GAS1 Budding Yeast)
UseTags2xHAExpressionMutationcontains promoter regionPromoterEndogenousAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GeNL
Plasmid#101002PurposeConstruct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only