We narrowed to 2,475 results for: T2A

1. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

   Plasmid

   #226515

   Purpose

   Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Cox8a(4x)-SNAP-tag2-T2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

   Plasmid

   #118593

   Purpose

   Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha/TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205550

   Purpose

   Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205551

   Purpose

   Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-I
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Puro-{CMV-CuO}>{PAX3::FOXO1}:T2A:mCherry

   Plasmid

   #236629

   Purpose

   Puromycin selected lentiviral construct with cumate-inducible PAX3::FOXO1

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Inserts

   PAX3::FOXO1 (FOXO1, PAX3 Human)
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV-CuO

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-Vgf-T2A-mCherry-CW3SL

   Plasmid

   #223661

   Purpose

   Expresses VGF and mCherry in a Cre-dependent manner

   Depositor

   Oscar Marin

   Article

   Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

   Insert

   Vgf-T2A-mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

   Plasmid

   #240799

   Purpose

   CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

   Depositor

   Hans Clevers

   Article

   Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

   Insert

   mNeonGreen-mCherry-CAAX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

    Plasmid

    #175547

    Purpose

    Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

    Plasmid

    #124494

    Purpose

    Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Caspase-1 cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid

    #175523

    Purpose

    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag11 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid

    #207805

    Purpose

    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

    Depositor

    Koen Breyne

    Article

    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

    Insert

    E-NoMi
    

    Use

    Lentiviral

    Tags

    3xFlag tag, copGFP, mCherry, and nanoluciferase

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

    Plasmid

    #51509

    Purpose

    Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV1

    Insert

    tdTomato-T2A-SypEGFP
    

    Use

    AAV

    Promoter

    phSyn1

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Available Since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

    Plasmid

    #197989

    Purpose

    For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    HSALNT0217290
    

    Use

    Lentiviral

    Tags

    lac

    Expression

    Mammalian

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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