We narrowed to 25,206 results for: CHI;

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pTJV1Sc-rpoB

Plasmid

#166978

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

Ec86 Retron (ECD_00831 )
lambda bet

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23101 and VanCC

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXTK025 [Origin_RK2-oriT_PartType1]

Plasmid

#229262

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType1

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

RK2-oriT Origin

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-6-GFP-P2A-NptII-9

Plasmid

#197741

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EGFP-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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sh-RNA hAtg13

Plasmid

#31971

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
ERroGFP_iE_C204Q_pCDNA3

Plasmid

#48629

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERroGFP

Tags

FLAG

Expression

Mammalian

Mutation

insertion E147B, C204Q

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCALNL-NaChBac

Plasmid

#125580

Purpose

Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

NaChBac

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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PDCD1-DEM (P1#5)

Plasmid

#227738

Purpose

Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting PDCD1-promoter

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LAG3-DEM (L3#1)

Plasmid

#227739

Purpose

Designer Epigenome Modifier (DEM) designed to target the LAG3-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

Plasmid

#213398

Purpose

transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

Depositor

Katrin Willig

Article

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

FingR for endogenous PSD95, transcriptinally regulated

Use

AAV

Tags

Citrine

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-ZoAl-RTD-3xF-ORF

Plasmid

#238486

Purpose

For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

DD644/645->AA

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-ZoAl-END-3xF-ORF

Plasmid

#238485

Purpose

For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

DD1041/1054->AA

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Sall4

Plasmid

#40797

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Sall4 (Sall4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pLicC-MBP-APETx2

Plasmid

#72668

Purpose

Periplasmic expression of APETx2 in E.coli

Depositor

Glenn King

Article

Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

Insert

APETx2

Tags

His tag, MBP, MalE signal sequence for periplasmi…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano50/pcDNA3

Plasmid

#51964

Purpose

mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano50

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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