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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 81,532 results for: ell

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Showing: 11201 - 11220 of 81532 results

  2. EBA175-bio

    Plasmid
    #47743
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBA175
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47743-plasmid-map-sequence-id-68751

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  3. RH2b-bio

    Plasmid
    #47778
    Purpose
    Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH2b (partial)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47778-plasmid-map-sequence-id-68797

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  4. RFP-(SUMO)10-(SIM)6

    Plasmid
    #122026
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 copies of human SUMO3 and 6 repeats of human SUMO interactive motif (SIM) from PIASx separated by (GGS)4 linker (SUMO3, PIAS2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    122026-plasmid-map-sequence-id-255492

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  5. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  6. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

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  7. 2xMARS

    Plasmid
    #205233
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205233-plasmid-map-sequence-id-404744

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  8. CD34-bio-His

    Plasmid
    #51647
    Purpose
    Expresses full-length Hematopoietic progenitor cell antigen CD34 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD34 (CD34 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51647-plasmid-map-sequence-id-78970

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  10. PX-TIR-GFP

    Plasmid
    #52737
    Purpose
    Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP and p40 Phox lipid binding domain
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. SLP2a-TIR-GFP

    Plasmid
    #52738
    Purpose
    Retroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    C2A domain of Murine SLP2a and GFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Available Since
    June 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187454
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187454-plasmid-map-sequence-id-367517

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Showing: 11201 - 11220 of 81532 results